单 位：
云南省林业有害生物防治检疫局;云南省林业科学院森林保护研究所;西南林业大学云南省森林灾害预警与控制重点实验室
关键词：
薇甘菊颈盲蝽;转录组;多聚半乳糖醛酸酶;Illumina测序
摘 要：
薇甘菊颈盲蝽Pachypeltis micranthus Mu et Liu(Hemiptera:Miridae)为世界性恶性杂草薇甘菊Mikania micrantha的自然天敌。在该天敌取食寄主的过程中,存在于其唾液中的多聚半乳糖醛酸酶作为重要的果胶水解酶在降解寄主植物细胞壁和引起寄主植物组织损伤中起着重要作用。然而,至今未有关于该天敌的任何基因信息被报道,本研究旨在获得其转录组数据,并基于该数据鉴定获得多聚半乳糖醛酸酶基因。采用Illumina测序技术进行转录组测定,共获得约5.2 Gbps的数据量,拼接得到57 739条非冗余基因(unigene)。通过BlastX注释,23 549条非冗余基因能与NCBI(National Center for Biotechnology Information)非冗余蛋白数据库中的已知蛋白相匹配,7 048、8 548和16 135条非冗余基因分别被成功的注释到GO(Gene Orthology)、COG(Clustes of Orthologous Groups)和KEGG(Kyoto Encyclopedia of Genes and Genomes)数据库中。基于构建的转录组数据库,共鉴定获得了24个多聚半乳糖醛酸酶基因,其中15个基因具有完整的开放阅读框。该转录组数据可为今后从分子水平研进一步研究薇甘菊颈盲蝽的生态学、生物学和生理学特性和机理提供了有价值的信息,鉴定获得的多聚半乳糖醛酸酶基因为深入研究揭示其在薇甘菊颈盲蝽与寄主互作中的作用奠定了基础。
译 名：
Transcriptomic analysis reveals polygalacturonase genes in Pachypeltis micranthus
作 者：
ZHU Jiaying;JI Mei;YANG Bin;ZE Sangzi;Key Laboratory of Forest Disaster Warning and Control of Yunnan Province,Southwest Forestry University;Institute of Forestry Protection,Yunnan Academy of Forestry;Bureau of Forestry Pest Control and Quarantine of Yunnan Province;
关键词：
Pachypeltis micranthus;;transcriptome;;polygalacturonase;;Illumina sequencing
摘 要：
The mirid bug,Pachypeltis micranthus Mu et Liu(Hemiptera:Miridae),is a natural enemy of the widespread invasive weed in the tropics,Mikania micrantha.To date,however,there is not any genetic information related to this species.Polygalacturonases(PGs) are one group of pectin hydrolases that are in the saliva of mirid bugs believed to be responsible for the digestion of plant cell wall and involved in causing visible plant injury.This study aims to obtain genetic data and reveal the putative PG genes of P.micranthus.The transcriptome of this species was sequenced using Illumina paired-end sequencing technology.Approximately 5.2 Gbps of sequences were generated,which were assembled into57 739 unigenes.Of these unigenes,23 549 non-redundant BlastX hits were identified.In total,7 048,8 548 and 16 135 unigenes were respectively subjected to Gene Orthology(GO),Clusters of Orthologous Groups(COG),and Kyoto Encyclopedia of Genes and Genomes(KEGG) annotations,respectivly.Based on the P.micranthus transcriptome,24 putative PG genes were identified,of which 15 were predicted to be full-length protein sequences.The findings provide valuable information to further exploration of this species from various ecological,biological and physiological aspects at the molecular level,and the role of PGs involved in the interaction between P.micranthus and its host.
