作 者：
张莉莉;宁冬雪;康丽君;寇芳;夏甜天;曹龙奎
关键词：
玉米皮渣;酸降解;响应面法;蒸汽爆破处理;还原糖
摘 要：
为提高湿法加工玉米淀粉产生的副产物玉米皮渣中还原糖的得率,以玉米皮渣为原料,研究蒸汽爆破处理原料、酸水解法制备还原糖的最优工艺条件,对硫酸体积分数、水解时间、水解温度和料液比4个因素分别进行单因素试验,根据单因素试验结果设计Box-Behnken试验,以还原糖含量为指标值,采用响应面分析法确定降解的最优工艺参数,通过离子色谱法分析水解产物的组分。结果表明:最优工艺参数为硫酸体积分数1.66%、水解时间1.5 h、水解温度120℃、料液比1∶10(g/m L),此条件下还原糖含量为54.61%,比未经蒸汽爆破处理的降解液中还原糖含量高出9.58%。降解液经离子色谱分析后发现主要含3种还原糖,分别为D-葡萄糖19.34?mg/m L、D-木糖16.01?mg/m L、L-阿拉伯糖10.37?mg/m L。同时对降解剩余物进行分析后发现,与原料相比蒸汽爆破酸解剩余物的纤维结构较疏松,裂解程度大,表面有孔洞和裂痕,说明蒸汽爆破酸解处理对纤维素、半纤维素及木质素的溶解力较强。这与两者降解液中还原糖含量结果相一致。
译 名：
Preparation of Reducing Sugar from Corn Bran by Steam Explosion Combined with Acid Hydrolysis: Process Optimization Using Response Surface Methodology and Analysis of Hydrolysis Degree
作 者：
ZHANG Lili;NING Dongxue;KANG Lijun;KOU Fang;XIA Tiantian;CAO Longkui;College of Food Science, Heilongjiang Bayi Agricultural University;Agri-Food Processing Development Centre of Heilongjiang;
关键词：
corn bran;;acid hydrolysis;;response surface methodology;;steam explosion;;reducing sugar
摘 要：
In order to increase reducing sugar yield from corn bran as a by-product of the production of corn starch by wet milling, the optimal conditions for preparation of reducing sugar from corn bran by steam explosion combined with acid hydrolysis were investigated in this research. First of all, the one-factor-at-a-time method was employed to examine the effects of sulphuric acid concentration, hydrolysis time, temperature and material-to-liquid ratio on reducing sugar content. Subsequently, the optimization of process parameters for improved reducing sugar content was performed using Box-Behnken design and response surface methodology. Hydrolysates were analyzed by ion chromatography. The results showed that the maximum reducing sugar content of 54.61% was obtained under the following optimal conditions: sulphuric acid concentration, 1.66%; hydrolysis time, 1.5 h; temperature, 120 ℃; and material-to-liquid ratio, 1:10(g/m L), which was increased by 9.58% as compared to that obtained without steam explosion pretreatment. Ion chromatography analysis indicated that the hydrolysate obtained was mainly composed of D-glucose(19.34 mg/m L), D-xylose(16.01 mg/m L) and L-arabinose(10.37 mg/m L). In comparison to the raw material, the fiber structure of the residue left after the preparation of reducing sugar was more loose and more significantly cracked, showing more holes and cracks on its surface, revealing that the cellulose, hemicellulose and lignin in corn bran can be well decomposed by successive steam explosion and acid hydrolysis. This result is in accordance with the reducing sugar content in the degradation solution obtained after both treatments.
