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Position: Home > Articles > Isolation,Identification and Pathogenicity Study of Fowl Adenovirus Group Ⅰ China Animal Health Inspection 2016,33 (8) 86-89+97

Ⅰ群禽腺病毒的分离鉴定及其致病性研究

作  者:
李晓林;王相芹;罗济冠;李明义
单  位:
山东信得科技股份有限公司
关键词:
Ⅰ群禽腺病毒;分离;鉴定;血清型;致病性
摘  要:
为了解Ⅰ群腺病毒(FAV-Ⅰ)在鸡群中的感染状况,对收集的178份疑似病料进行鸡胚病变、细胞病变特征分析,利用RT-PCR、基因测序和BLAST进行病毒的分离鉴定,成功分离出25株FAV-Ⅰ,其中20株与血清4型同源性较高,5株与血清8型同源性较高。动物回归试验结果表明,攻毒鸡的临床症状和病理变化与自然发病鸡基本一致。
译  名:
Isolation,Identification and Pathogenicity Study of Fowl Adenovirus Group Ⅰ
作  者:
Li Xiaolin;Wang Xiangqin;Luo Jiguan;Li Mingyi;Shandong Sinder Technology Co.,Ltd.;
关键词:
fowl adenovirus group Ⅰ;;isolation;;identification;;serotypes;;pathogenicity
摘  要:
In order to evaluate infection status of fowl adenovirus group Ⅰ(FAV-Ⅰ)in chickens,characteristic analysis of embryo lesion and cytopathic effect was carried out towards the collected 178 suspected samples.Virus was isolated and identified by RT-PCR,gene sequencing and BLAST test,and 25 strains of FAV-Ⅰ were successfully isolated.Among all of them,20 strains have high homology with serotype 4,and 5 strains have high homology with serotype 8. Results of animal regression test indicated that the clinical symptoms and necropsy lesions of challenging chickens were consistent with natural infection.

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