Position: Home > Articles > Polymorphisms of BMP15 Gene Exon 1 and Its Association with Egg Quality in Sizhou Chicken
China Animal Husbandry & Veterinary Medicine
2019
(12)
3690-3697
思州鸡BMP15基因第1外显子多态性及其与蛋品质的关联分析
作 者：
李洪林;李维;张福平;祖盘玉;牟腾慧;刘洋;龙广丽;李开阳;林家栋
单 位：
贵州省毕节市赫章县动物卫生监督所;贵州大学动物科学学院高原山地动物遗传育种与繁殖省部共建教育部重点实验室;贵州省畜禽遗传资源管理站
关键词：
思州鸡;BMP15基因;多态性;蛋品质
摘 要：
研究旨在探讨思州鸡骨形态发生蛋白15(BMP15)第1外显子多态性及其与蛋品质的相关性。采用PCR产物直接测序法检测思州鸡BMP15基因第1外显子测序,将测序结果用DNAStar软件校正;利用Excel 2007统计基因型频率数据,并进行卡方适合性检验;通过SPSS 18.0软件对BMP15基因不同基因型与思州鸡蛋品质间的关联性进行分析。结果显示,思州鸡BMP15基因第1外显子存在4个SNPs位点:397C/T、473A/G、603C/T和612C/T,均处于Hardy-Weinberg非平衡状态(P<0.05);397C/T和603C/T位点的多态信息含量(PIC)分别为0.3488和0.3299,属于中度多态(0.250.05);612C/T位点对思州鸡蛋长径有显著影响(P<0.05),提示BMP15基因第1外显子对鸡蛋品质中蛋白高度、蛋长径具有较大的遗传效应。结果表明,BMP15基因可能是影响思州鸡蛋品质的基因之一,可作为思州鸡蛋白高度、蛋长径等蛋品质性状的分子标记。
译 名：
Polymorphisms of BMP15 Gene Exon 1 and Its Association with Egg Quality in Sizhou Chicken
作 者：
LI Honglin;LI Wei;ZHANG Fuping;ZU Panyu;MU Tenghui;LIU Yang;LONG Guangli;LI Kaiyang;LIN Jiadong;Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau,Mountainous Region,Ministry of Education,College of Animal Sciences,Guizhou University;Guizhou Management Station of Livestock Genetic Resources;Animal Health Supervision Institute of Hezhang County in Bijie City of Guizhou Province;
关键词：
Sizhou chicken;;BMP15 gene;;polymorphism;;egg quality
摘 要：
This study was aimed to explore the polymorphisms of BMP15 gene exon 1 and its association with egg quality in Sizhou chicken.The direct sequencing of PCR products was adopted to detect the polymorphism of BMP15 gene exon 1 in Sizhou chicken.After direct sequencing,sequence adjusting were conducted using DNAStar software.Data of genotype frequency was counted by Excel 2007 and analyzed by χ~2 test.The association of genotype and egg quality were analyzed with SPSS 18.0.The results showed that BMP15 gene exon 1 in Sizhou chicken had four SNPs:397 C/T,473 A/G,603 C/T and 612 C/T,which were not in Hardy-Weinberg equilibrium(P<0.05);The polymorphic information content(PIC) of 397 C/T and 603 C/T loci were 0.3488 and 0.3299,respectively,belonging to moderate polymorphism(0.250.05);The 612 C/T locus had a significant influence on egg length of Sizhou chicken,respectively(P<0.05).This results suggested that BMP15 gene exon 1 had a larger genetic effect on the protein height and egg length in Sizhou chicken.BMP15 gene might be one of the genes affecting the egg quality in Sizhou chicken,and could be used as a molecular marker for egg quality traits such as protein height and egg length and diameter in Sizhou chicken.
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