Position: Home > Articles > A new method for identifying chitin-decomposing microorganisms
Plant Protection
2010,36
(4)
163-166
快速、准确鉴别产几丁质酶菌株的新方法
作 者：
胡晓;张敏;刘彭强;邓秋蕾;舒凯
单 位：
四川农业大学农学院植物病理系
关键词：
几丁质分解菌;刚果红;鉴别培养基
摘 要：
使用几丁质平板法筛选具有几丁质酶活性的细菌时,由于几丁质平板本身趋于透明,而降解环也为透明色,不易观察到明显的抑菌圈,故在筛选几丁质降解菌时,其观察结果受人为因素影响较大。本文首次使用刚果红染色的方法,对几丁质平板进行染色,降解环为浅红色,而未降解部分为深红色;结果表明,降解环的直径大小随着接种时间的延长而逐步增大,且降解环的大小可反映酶活大小。因此,此法可以直观、清晰、准确地对具有几丁质酶活性的菌株进行筛选,在相关菌株的筛选中具有较大的潜在应用价值。
译 名：
A new method for identifying chitin-decomposing microorganisms
作 者：
Hu Xiao,Zhang Min,Liu Pengqiang,Deng Qiulei,Shu Kai(Department of Plant Pathology,Sichuan Agricultural University,Ya'an 625014,China)
关键词：
chitin-decomposing microorganism;Congo red;differential medium
摘 要：
When screening the chitin-decomposing microorganisms with chitin-flat-band method,we found the decomposing ring was translucent,with similar color to chitin-flat-bands,which has led to failure and difficulty in filtering decomposing microorganisms.In order to overcome these limitations,the present study developed a novel strategy with Congo red differential medium which could conveniently isolate chitin-decomposing microorganisms from other microorganisms.The results showed that the chitin-decomposing microorganisms cultivated by chitinflat-band were surrounded by some distinct red zones in the medium after Congo red coloration,and the size of hydroids had strong relationships with culture time and chitinase activity,suggesting it was a simple and visual method for differentiating the chitin-decomposing microorganisms containing chitinase from other microorganisms.The potential for application of this method was also discussed.
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