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Position: Home > Articles > Construction and Identification of a cDNA Expression Library from Dendrobium nobile Acta Horticulturae Sinica 2006,33 (4) 895-897

金钗石斛花芽cDNA表达文库的构建及鉴定

作  者:
庄军平;黄胜琴;潘舒群;叶庆生
单  位:
华南师范大学生命科学学院广东省植物发育生物工程重点实验室
关键词:
金钗石斛;花芽;cDNA表达文库
摘  要:
cDNA文库是分离、筛选基因的重要平台。利用SMARTTMcDNAlibraryconstruction技术,成功构建了金钗石斛花芽cDNA表达文库。结果表明该文库的克隆数为3·02×105pfu,插入片段的大小在0·5~2·5kb范围,插入片段的重组率接近100%,说明所构建的cDNA文库质量较好。该文库的构建为克隆与金钗石斛花芽分化相关功能基因奠定了基础。
译  名:
Construction and Identification of a cDNA Expression Library from Dendrobium nobile
作  者:
Zhuang Junping, Huang Shengqin, Pan Shuqun, and Ye Qingsheng (College of Life Science, South China Normal University, Guangdong Key Laboratory of Biotechnology for Plant Development, Guangzhou, Guangdong 510631,China)
关键词:
Dendrobium nobile;Flower bud;cDNA expression library
摘  要:
A cDNA expression library from the flower bud of Dendrobium nobile was constructed into pcDNA3.0 eukaryotical expression plasmid used SMART~ TM cDNA library construction protocol. The result showed that the cDNA expression library contained 3.02×10~5 pfu,the size of the inserts ranges from 0.5 kb to 2.5 kb, and the percentage of positive clones was almost 100%. All of the above mentioned have answered for the general requirements of a cDNA library. This provides a base for cloning of important genes related to flower bud differentiation in Dendrobium nobile.

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