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当前位置: 首页 > 文章 > 鹅副黏病毒与新城疫病毒对鸡胚和鹅胚成纤维细胞的感染性分析 动物医学进展 2005,26 (2) 76-79
Position: Home > Articles > Pathogenic Analysis of NDV and GPMV to Embryo Fibroblasts Progress in Veterinary Medicine 2005,26 (2) 76-79

鹅副黏病毒与新城疫病毒对鸡胚和鹅胚成纤维细胞的感染性分析

作  者：
单松华;谢爱织;邹键;姚龙涛
单  位：
上海出入境检验检疫局;中国科学院上海生命研究院生物化学与细胞生物学研究所;上海市奉贤区畜牧兽医站
关键词：
鹅副黏病毒;新城疫病毒;胚胎成纤维细胞;噻唑蓝显色;致病性
摘  要：
用鸡新城疫病毒F48E9,Komarov, LaSota,V4/66株和鹅副黏病毒SF02株分 别感染鸡胚和鹅胚成纤维细胞,用MTT显 色方法检测存活细胞数目。结果表明,不同 毒力新城疫病毒致细胞病变的作用不一样, 鸡新城疫强毒F48E9株和鹅副黏病毒SF02 株均引起两种细胞几乎全部死亡,感染动物 时,SF02与NDV强毒株的致病性有显著的 区别,但感染细胞时,二者并无明显差别; NDV与GPMV在感染水禽时的致病性差异 并不是因为诸如细胞膜受体等细胞水平的因 素不同造成的,可能与干扰素途径有关。
译  名：
Pathogenic Analysis of NDV and GPMV to Embryo Fibroblasts
作  者：
SHAN Song-hua~1,XIE Ai-zhi~1,ZOU Jian~2,YAO Long-tao~3(1.Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai,200135,China;2.Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences,Shanghai,200031,China;3.Shanghai Fengxian Veterinary Station,Fengxian,Shanghai,201400,China)
关键词：
GPMV;NDV;embryo fibroblast;MTT;pathogenicity
摘  要：
It has been shown that there is a significant difference between goose paramyxovirus (GPMV),designated as SF02, and Newcastle disease virus (NDV) in the host pathogenicity. Pathogenicities difference in culture cells between GPMV and NDV were investigated. Chicken and goose embryo fibroblasts were infected with NDV strains F48E9 (velogenic), Komarov (mesogenic), La Sota (lentogenic), V4/66 (avirulent) and GPMV SF02. Live cells were detected by using MTT method. The results showed CPE were related to Pathogenicites of NDV and there were not significant distinction between the pathogenicities of NDV and GPMV to embryo fibroblasts.The cell membrane receptor may not due to pathogenic difference between NDV and GPMV,but ratate to the interferons.

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