作 者：
康润敏;李瑶;吕学斌;姬高升;应三成;曾凯;李琰;殷明郁
关键词：
丫杈猪;青裕猪;乌金猪;盲肠;微生物多样性;纤维素消化能力
摘 要：
为了研究中国特有地方猪种丫杈猪、青裕猪和乌金猪盲肠微生物菌落的组成,揭示地方猪种耐粗饲特性的机理,本试验采用16SrDNA扩增子测序技术比较分析了体重90kg左右的丫杈猪、青裕猪和乌金猪盲肠微生物菌群的多样性。结果显示,在门水平上3个品种猪的核心菌群均为拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes),在属的水平上均为普雷沃菌属(Prevotella)和拟杆菌属(Bacteroides);在3个猪种的盲肠中均发现了与降解纤维素相关的细菌,其中纤维杆菌属(Fibrobacter)和梭菌属(Clostridium)在丫杈猪盲肠中的数量显著高于青裕猪和乌金猪(P<0.05),普雷沃氏菌属(Prevotella)在丫杈猪盲肠内的数量显著高于乌金猪(P<0.05),拟杆菌属(Bacteroides)在青裕猪盲肠内的数量显著高于丫杈猪(P<0.05),螺旋体门(Spirochaetes)在青裕猪盲肠内的数量显著高于乌金猪(P<0.05)。结果表明,3个品种猪盲肠内微生物菌群分布的种类相似度高,但分布规律和数量存在显著差异;对与纤维素消化相关菌群的分析表明,丫杈猪对纤维消化的能力是3个品种中最强的,其次是青裕猪,乌金猪最弱。
译 名：
Analysis of the Cecum Microbial Diversity of Different Pig Breeds by Illumina Amplicon Sequencing of 16SrDNA Tag
作 者：
KANG Run-min;LI Yao;LV Xue-bin;JI Gao-sheng;YING San-cheng;ZENG Kai;LI Yan;YIN Ming-yu;Animal Breeding and Genetics Key Laboratory of Sichuan Province,Sichuan Animal Science Academy;Key Laboratory of Bio-resources and Ecoenvironment of Ministry of Education,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,College of Life Sciences,Sichuan University;
单 位：
Animal Breeding and Genetics Key Laboratory of Sichuan Province,Sichuan Animal Science Academy%Key Laboratory of Bio-resources and Ecoenvironment of Ministry of Education,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,College of Life Sciences,Sichuan University
关键词：
Yacha pig;;Qingyu pig;;Wujin pig;;cecum;;microbial diversity;;cellulose digest ability
摘 要：
To study the diversity and composition of the bacterial community from cecum samples from Yacha pig,Qingyu pig and Wujin pig,the investigation was designed to reveal mechanism of roughage resistance.Illumina amplicon sequencing of 16 SrDNA Tag was used to analyze the cecum microbial diversity of Yacha pig,Qingyu pig and Wujin pig at 90 kg liveweight.The results indicated that the core flora of the three breeds were Bacteroidetes and Firmicutes at the phylum level and Prevotella and Bacteroides at the genus level.Meanwhile bacteria associatedwith cellulose decomposition were found in all three breeds,in which the number of Fibrobacter and Clostridiumin Yacha pig were significantly higher than that in Qingyu pig and Wujin pig(P<0.05),and the number of Prevotellain Yacha pig was significantly higher than that in Wujin pig(P<0.05).The number of Bacteroidesin Qingyu pig was significantly higher than that in Yacha pig(P<0.05).The number of Spirochaetes in Qingyu pig was significantly higher than that in Wujin pig(P<0.05).It could conclud that the composition of the cecum bacteria community was similar in above pig breeds,but there were significant differences in distribution and quantity.According to analysis of microbial related to cellulose digestibility,Yacha pig was the strongest among the three breeds,followed by the Qingyu pig and Wujin pig.
