关键词：
苹果砧木组培苗;PEG-6000胁迫;抗旱性评价
摘 要：
【目的】为了提供苹果耐旱育种及栽培材料,【方法】以5种苹果砧木组培苗为试材,PEG-6000作为胁迫剂,模拟室外干旱进行处理,运用模糊隶属函数抗旱性综合分析,比较了5个苹果砧木组培苗的耐旱性。【结果】结果表明,在PEG-6000胁迫条件下,不同苹果砧木的叶绿素含量总体均呈下降趋势,叶片的相对电导率和MDA含量随PEG-6000胁迫质量分数的升高逐渐升高。在整个胁迫过程中,各苹果砧木组培苗叶片内蛋白质含量随胁迫质量分数的升高而增大。SOD活性随PEG-6000胁迫质量分数的升高呈先升高后下降趋势,且在20%PEG-6000胁迫条件下SOD活性达到了最大。POD活性随胁迫质量分数的升高呈现出逐渐升高的趋势。【结论】5种苹果砧木的抗旱性顺序依次为:新疆野苹果>平邑甜茶>八棱海棠>‘M26’>‘M9’。
译 名：
Drought resistance of different apple rootstocks in vitro
作 者：
ZHANG Jing,ZHAO Liang-ming,ZOU Zhi-rong (College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100 China)
关键词：
Apple rootstocks;PEG-6000 stress;Drought resistance evaluation
摘 要：
【Objective】 The study was to provide drought tolerant apple lines for breeding and cultivation practice.【Method】 Tissue cultured shoots of five apple rootstocks were treated with PEG-6000,simulating field drought stress conditions.Drought tolerance of the shoots was evaluated by meta-analysis using fuzzy membership function.【Result】 The results showed that the chlorophyll content displayed a decreasing tendency under PEG-6000 stress,whereas the relative electric conductivity and malondialdehyde(MDA) content in leaf increased gradually with the increase of PEG-6000 concentration.In the stress treatment,the protein content in leaf of all rootstocks increased with the PEG-6000 concentration.Superoxide dismutases(SOD) activity first increased and then declined with increasing PEG-6000 concentration,with a peak value at 20% PEG-6000.However,the POD activity went up gradually in all the rootstocks with the increase of drought stress strength.【Conclusion】 The drought tolerances of the five rootstocks ranked as follows: Malus sieversii>M.hupehensis>M.micromalus>'M26'>'M29'.
