当前位置: 首页 > 文章 > 不同SSR标记检测技术及其在花生栽培种遗传多样性分析中的应用 植物遗传资源学报 2014,15 (1) 96-105
Position: Home > Articles > Different SSR Detection Techniques and their Application in Genetic Diversity Analysis of Peanut( Arachis hypogaea L.) Cultivars Journal of Plant Genetic Resources 2014,15 (1) 96-105

不同SSR标记检测技术及其在花生栽培种遗传多样性分析中的应用

作  者:
王燕龙;单雷;付春;徐平丽;姜言生;柳展基;曲志才;唐桂英
单  位:
曲阜师范大学生命科学学院;山东省潍坊市农业科学院;山东省农业科学院高新技术研究中心/山东省作物遗传改良与生态生理重点实验室
关键词:
花生;SSR;银染法;荧光检测法;遗传多样性
摘  要:
以85份花生栽培种为材料,分别应用银染法和荧光检测法检测9对SSR引物的扩增产物。比较结果显示,荧光检测法具有灵敏度高、检测结果准确、效率高等优点。聚类分析表明,银染法与荧光检测法分别能够区分74个、82个花生品种,并分别聚成8个、9个类群;荧光检测法的聚类结果虽然反映的品种间遗传多样性较低,但与品种类型、产地及其亲缘关系相关程度更高,表明荧光检测数据更精确、可靠。遗传多样性分析发现,地方品种的遗传多样性指数最高,其次为多粒型育成品种,表明我国地方品种和多粒型育成品种蕴藏了丰富的优异性状,有利于对其挖掘和利用。
译  名:
Different SSR Detection Techniques and their Application in Genetic Diversity Analysis of Peanut( Arachis hypogaea L.) Cultivars
作  者:
WANG Yan-long;SHAN Lei;FU Chun;XU Ping-li;JIANG Yan-sheng;LIU Zhan-ji;QU Zhi-cai;TANG Gui-ying;Hi-Tech Research Center,Shandong Academy of Agricultural Sciences / Shandong Provincial Key Laboratory of Crop Genetic Improvement Ecology and Physiology;College of Life Science,Qufu Normal University;Weifang Academy of Agricultural Sciences;
关键词:
peanut;;SSR;;silver-staining;;fluorescence detection;;genetic diversity
摘  要:
In the present study,we assessed 9 SSRs products of 85 peanut cultivars using silver-staining method and fluorescent detection assay. The results showed fluorescent detection assay was more sensitive,accurate,and efficient than silver-staining method. Cluster analysis indicated fluorescent detection assay was able to separate 82 peanut cultivars into 9 clusters,while silver-staining method separated 74 cultivars into 8 clusters. The clusters from fluorescent detection assay were more closely related to the types,origins,and pedigrees of the peanut cultivars. Genetic diversity analysis showed the peanut landraces had the highest genetic diversity index,followed by the Valencia type peanut cultivars,suggesting the landraces and Valencia type cultivars harboring a broad genetic variation.

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