作 者：
李俊营;詹凯;毕善辉;刘伟;韩涛;李绍全
单 位：
安徽省农业科学院畜牧兽医研究所;安徽皖西麻黄鸡禽业有限公司;安徽农业大学生命科学学院
关键词：
OCX-32基因;淮南麻黄鸡;蛋品质;安徽省
摘 要：
【目的】研究OCX-32基因在淮南麻黄鸡、五华鸡、淮北麻鸡和皖南三黄鸡等4个安徽省地方鸡品种中的遗传多态性及OCX-32基因与淮南麻黄鸡蛋品质的相关性,为揭示这些群体的遗传特征提供基础数据。【方法】采用PCR-RFLP技术,对淮南麻黄鸡、五华鸡、淮北麻鸡和皖南三黄鸡4个地方鸡品种的OCX-32基因外显子4进行遗传多态性研究,并对外显子4遗传多态性与淮南麻黄鸡蛋品质的相关性进行分析。【结果】OCX-32基因外显子4存在A494C1个单核苷酸多态性位点。该位点在安徽省4个地方鸡品种中的基因频率和基因型频率分布差异不大,C等位基因频率较高。4个地方鸡品种群体在A494C位点处于Hardy-Weinberg平衡状态,均表现为中度多态。相关分析结果表明,OCX-32基因外显子4的A494C位点基因多态性与淮南麻黄鸡的开产蛋质量、30周龄蛋质量、蛋白高度和哈氏单位显著相关(P<0.05);CC基因型的开产蛋质量和30周龄蛋质量显著高于AA基因型,AA和CC基因型的蛋白高度显著高于AC基因,AA基因型的哈氏单位显著高于AC基因型(P<0.05)。【结论】OCX-32基因与淮南麻黄鸡蛋品质存在一定的相关性,可作为发掘淮南麻黄鸡蛋品质的分子育种标记。
译 名：
Polymorphisms of exon 4 of OCX-32 gene in four indigenous chicken breeds in Anhui Province and its relationship with egg quality of Huainan partridge chicken
作 者：
LI Jun-ying;ZHAN Kai;BI Shan-hui;LIU Wei;HAN Tao;LI Shao-quan;Institute of Animal Husbandry and Veterinary Medicine,Agricultural Academy of Anhui Province;College of Life Science,Anhui Agricultural University;Anhui Wanxi Mahuangji Poultry Co.,Ltd;
关键词：
OCX-32gene;;Huainan partridge chicken;;egg quality;;Anhui Province
摘 要：
【Objective】This study was aimed to analyze the genetic variations of OCX-32gene of Huainan partridge chicken,Wuhua chicken,Huaibei ma chicken and Wannan sanhuang chicken in Anhui Province,and investigate the relationship between OCX-32gene and egg quality of Huainan partridge chicken in order to reveal these populations' genetic characteristics and provide data for marker-assisted selection of egg quality in future.【Method】The polymorphisms of exon 4of OCX-32gene were analyzed by PCRRFLP technique in Huainan partridge chicken,Wuhua chicken,Huaibei ma chicken,Wannan sanhuang chicken and the effects of genotypes of exon 4in OCX-32gene on egg quality traits of Huainan partridgechicken were estimated.【Result】A mutation of A to G was found in the exon 4of OCX-32gene(A494C). The genotype and allele frequencies in the four domestic breeds varied similarly and allele C was higher. The four populations were at Hardy-Weinberg equilibrium at A494Csite(P<0.05)and showed moderate polymorphism.Significant effects of A494Cin the exon 4of OCX-32gene on egg weight at first laying,egg weight at 30th week,albumen height and Haugh unit were observed in Huainan partridge chicken population(P<0.05).The chickens with CC genotype had higher egg weight of first laying and egg weight at 30th week than that of genotype AA genotype(P<0.05).The albumen height of AA and CC genotype were higher than that of AC genotype(P<0.05),and Haugh unit of AA genotype was higher than that of AC genotype in Huainan partridge chicken(P<0.05).【Conclusion】OCX-32gene affected egg quality and could be used as molecular breeding marker for egg quality of Huainan partridge chicken.
