Expression of Feline Interferon ω in Bombyx m ori Baculovirus Expression System and Determination of Its Antiviral Activity

Chen Xiaoyan;Li Haoyang;Hu Xiaoyuan;Lü Yanna;Li Yinü;Zhang Zhifang;Yi Yongzhu;Jiangsu University of Science and Technology;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences;The Sericultural Research Institute,Chinese Academy of Agricultural Sciences;

Feline interferon ω;;Bombyx mori baculovirus expression system;;Antiviral activity

The optimized feline interferon ω gene Fe IFN-ω2 and Fe IFN-ω9 were artificially synthesized and cloned into baculovirus transfer vector p VL1393 to construct the recombinant plasmid under control of polyhedron gene's promoter.The recombinant plasmids were co-transfected into Bm N cells with orf1629-defective Bm-Bacmid DNA for homologous recombination. The obtained recombinant virus was used to infect silkworm( Bombyx mori) larvae of the 5th instar to express feline interferon ω in silkworm bioreactor. The recombinant feline interferon ω expressed in silkworm hemolymph had antiviral activity revealed by mini-cytopathic effect inhibition assay using the CRFK cell / VSV* GFP system. The antiviral activity of expression products of recombinant Fe IFN-ω2 could reach 6. 3 × 105 U / m L,while that of recombinant Fe IFN-ω9 was low. This study provides an experimental basis for producing recombinant feline interferon ω biological agents using silkworm bioreactor.