作 者：
俎峰;赵凯琴;张云云;田正书;刘亚俊;奚俊玉;束正齐;符明联
单 位：
云南省临沧市农业技术推广站;丽江市玉龙县农业技术推广中心;云南省农业科学院经济作物研究所
关键词：
甘蓝型油菜;花期;生育期;QTL定位;SNP芯片
摘 要：
【目的】对甘蓝型油菜花期和生育期QTL进行定位,为精细定位和克隆早花基因及开展分子标记辅助早熟油菜品种选育提供理论依据。【方法】以极早熟甘蓝型油菜G28、甘蓝型油菜H008及以二者为亲本构建的175个F1DH株系为材料,利用甘蓝型油菜60K SNP芯片分型技术绘制高密度遗传连锁图谱,并采用完备复合区间作图法对2016─2017年度丽江和临沧2个生长环境下甘蓝型油菜的花期(FT)和生育期(MT)田间调查数据进行QTL扫描分析。【结果】F1DH株系花期与生育期具有较明显的超亲现象,表明双亲材料控制花期和生育期的位点不同。F1DH株系在丽江生长环境下花期与生育期相关系数为0.63,在临沧生长环境下二者相关系数为0.79,即花期与生育期呈较高的正相关。利用SNP芯片构建的高密度遗传连锁图谱共包含19条连锁群,7601个SNPs位点,总长3838.2 c M。在丽江和临沧2个生长环境下共检测到6个花期QTL和5个生育期QTL,分布于A02、A07、C02、C03、C06、C07和C09连锁群上,可分别解释2.96%～17.40%和4.98%～11.82%的遗传变异。花期QTL qFTA02-1和qFTC03-2在2生长个环境下均可检测到,加性效应值相反,其中qFTA02-1具有最高的LOD值(20.43)、贡献率(17.40%)和加性效应值(3.27 d),且与生育期QTL qMTA02-1置信区间重叠,是最主要的花期主效QTL;qFTC03-2为次要的花期主效QTL。在qFTA02-1置信区间内发现2个拟南芥花期调控关键基因FLC和FY的油菜同源基因拷贝BnaA02g00370D和BnaA02g01670D。【结论】花期主效QTL qFTA02-1和qFTC03-2可用于分子标记辅助选育早熟油菜品种。BnaA02g00370D和BnaA02g01670D可能为qFTA02-1置信区间内控制甘蓝型油菜花期性状的目标基因。
译 名：
QTL mapping of flowering time and maturity time in Brassica napus L.
作 者：
ZU Feng;ZHAO Kai-qin;ZHANG Yun-yun;TIAN Zheng-shu;LIU Ya-jun;XI Jun-yu;SHU Zheng-qi;FU Ming-lian;Industrial Crops Institute,Yunnan Academy of Agricultural Sciences;Agricultural Technology Extension Station in Lincang City;Yulong Centre of Agricultural Technique,Lijiang;
关键词：
Brassica napus L.;;flower time;;maturity time;;QTL mapping;;SNP array
摘 要：
【Objective】The study aimed to explore QTL mapping of flower time(FT)and maturity time(MT)of Brassica napus L. to obtain available information for finely mapping and cloning the gene related with early blooming trait and breeding of early-maturing rape variety assisted by molecular markers.【Method】G28,a super premature B. napus inbred line and H008,a B. napus inbred line,and 175 F1 DH lines with the above lines as parents were used as research materials.B. napus 60 K SNP array were used to construct a high density linkage map. The field survey data of FT and MT were collected from Lijiang and Lincang in 2016-2017. QTL identification of FT and MT was conducted using the model of complete composite interval mapping.【Result】The FT and MT of F1 DH line population presented an obvious transgressive segregation. It indicated that the loci that controlling FT and MT traits were different between their parents. Correlation coefficient of F1 DH line population between FT and MT was 0.63 in Lijiang and 0.79 in Lincang,indicating high positive correlation between FT and MT. High density linkage map established by SNP array contained 19 linkages,a total of7601 SNP sites covering 3838.2 cM. Six and five QTLs were detected related with FT and MT traits respectively in Lijiang and Lincang,distributing in A02,A07,C02,C03,C06,C07 and C09 linkages,which could explain 2.96%-17.40%and 4.98%-11.82% of the genetic effects respectively. The FT QTL,qFTA02-1 and q FTC03-2 were both detected in two environments,having opposite additive effects. The LOD value,contribution rate and additive effects of q FTA02-1 were as high as 20.43,17.40% and 3.27 d,respectively,which were all higher than those of qFTC03-2. In addition,the confidential interval of FT QTL qFTA02-1 overlapped with MT QTL qMTA02-1. q FTA02-1 was the major QTL at FT,and qFTC03-2 was the secondary major QTL at FT. Further analysis indicated that there were two candidate genes,BnaA02 g00370 D and BnaA02 g01670 D in confidential interval of qFTA02-1,which were homologous copies of FLC and FY genes controlling FT in Arabidopsis.【Conclusion】The major QTL at FT,qFTA02-1 and qFTC03-2,can be used in molecular markerassisted selection of early mature rape breeding,and the candidate genes BnaA02 g00370 D and BnaA02 g01670 D can be the target genes controlling the FT trait of B. napus in confidence interval of qFTA02-1.
