GAO Ye;CHENG Youmin;ZHAO Linhu;NIU Chunhong;YAN Yuqing;YAN Hailong;CHEN Yulin;Shanxi Datong Medical College;College of Animal Science and Technology, Northwest A&F University;

arachidonic acid;;VDR;;cashmere goat;;dermal papilla cells

To investigate the influence of arachidonic acid(AA) on the proliferation of dermal papilla cells(DPCs) in cashmere goats and seek the mechanism of AA on the expression of vitamin D receptor gene(VDR), DPCs were cultured in vitro and treated with different concentrations of AA for different periods. The viability of DPCs survival rate was detected by CCK8, the expression level of VDR was detected by immunofluorescence staining and RT-PCR, the mRNA expression level of hair follicle cycle related genes(IGF1, Noggin, b-catenin and Lef1) were detected by RT-PCR. Results showed the treatment with 10~(-7) M of AA could significantly increase the the activity of DPCs, and significantly increase the expression of VDR mRNA and fluorescent protein in DPCs(P<0.05). AA significantly increased the expression of IGF1 and Lef1 genes in DPCs(P<0.05), while IGF1 was not affected by AA in VDR gene-deficient DPCs. This study proved AA could promote the proliferation of DPCs and the expression of VDR, IGF1 and Lef1. The effect of AA on the expression of IGF1 in hair follicle is regulated by VDR. This research will provide new ideas for the regulation of hair follicle formation and development of cashmere goats.