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当前位置: 首页 > 文章 > 基于猪圆环病毒2型病毒样颗粒的间接ELISA抗体检测方法的建立 中国兽医科学 2022,52 (1) 56-61
Position: Home > Articles > Establishment of indirect ELISA antibody detection method based on porcine circovirus type 2 virus-like particles Chinese Veterinary Science 2022,52 (1) 56-61

基于猪圆环病毒2型病毒样颗粒的间接ELISA抗体检测方法的建立

作  者：
敬晓棋;王运航;张韵;白满元
单  位：
中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室;榆林学院生命科学学院榆林市动物疾病诊断与检测重点实验室
关键词：
猪圆环病毒2型;病毒样颗粒;间接ELISA;敏感性;特异性
摘  要：
为了建立一种更加安全、可靠的猪圆环病毒2型(PCV2)抗体检测方法,以实验室前期通过大肠杆菌表达系统获取的PCV2病毒样颗粒(VLPs)为包被抗原,建立了一种间接ELISA方法。用建立的间接ELISA方法检测272份来自不同猪场的血清,并与不同间接ELISA试剂盒的检测结果进行比较。结果表明,基于PCV2 VLPs的间接ELISA的特异性和敏感性分别为100.00%和91.67%。其与Ceditest CIRC Ig G ELISA试剂盒(Cedi-Diagnostics B.V.)和猪圆环病毒2型ELISA抗体检测试剂盒(科前)的符合率分别为97.56%和95.12%。因此,基于PCV2 VLPs的间接ELISA可以有效地用于猪血清样本中PCV2抗体的检测,为PCV2的流行病学调查及PCV2 VLPs疫苗的免疫效果评价提供了新的选择。
译  名：
Establishment of indirect ELISA antibody detection method based on porcine circovirus type 2 virus-like particles
作  者：
JING Xiao-qi;WANG Yun-hang;ZHANG Yun;BAI Man-yuan;Yulin Key Laboratory for Diagnosis and Detection of Animal Disease/Life Science College,Yulin University;State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;
关键词：
porcine circovirus type 2;;virus-like particles;;indirect ELISA;;sensitivity;;specificity
摘  要：
In order to establish a more safe and reliable method for detection of porcine circovirus type 2(PCV2) antibody,an indirect ELISA method was established with PCV2 virus-like particles(VLPs)obtained by Escherichia coli expression system as coating antigen.A total of 272 sera from different pig farms were detected by the established indirect ELISA method,and the results were compared with those of different indirect ELISA kits.The results showed that the specificity and sensitivity of established indirect ELISA were 100.00% and 91.67%,respectively.The coincidence rates with Ceditest CIRC Ig G ELISA Kit(Cedi-Diagnostics B.V.) and PCV2 ELISA antibody detection kit(Keqian) were 97.56%and 95.12%,respectively.Therefore,indirect ELISA based on PCV2 VLPs can be effectively used to detect PCV2 antibodies in pig serum samples,which provides a new choice for the epidemiological investigation of PCV2 and the evaluation of the immune effect of PCV2 VLPs vaccine.

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