Position: Home > Articles > Chemotaxis response of Rhizoctonia solani and Sclerotinia schinsengto total ginsenosides
Journal of Northwest A & F University(Natural Science Edition)
2016,44
(5)
200-204+214
立枯丝核菌和菌核菌对人参总皂苷化学趋向性的响应
作 者：
张爱华;匙坤;许永华;孙佳明;雷锋杰
单 位：
长春中医药大学生物工程研发中心;吉林农业大学中药材学院
关键词：
人参立枯丝核菌;人参菌核菌;人参总皂苷;化学趋向性
摘 要：
【目的】探讨人参立枯丝核菌和菌核菌对人参总皂苷化学趋向性的响应。【方法】采用平板法测定人参立枯丝核菌和菌核菌在不同人参总皂苷质量浓度(200,20,2,0.2mg/L)、温度(10,15,20,25,30℃)和pH(5,6,7,8)下的化学趋向性响应,以及初生菌丝受此趋化影响下的生长状况。【结果】人参立枯丝核菌对25℃、pH=6、质量浓度为2mg/L的人参总皂苷表现出较强的正趋向性,而人参菌核菌则对15℃、pH=7、质量浓度为2mg/L的人参总皂苷表现出较强的正趋向性,趋化移动指数(CMI)分别为0.136和0.149。【结论】人参总皂苷作为趋化因子能够诱导人参立枯丝核菌和菌核菌产生化学趋向性响应。
译 名：
Chemotaxis response of Rhizoctonia solani and Sclerotinia schinsengto total ginsenosides
作 者：
ZHANG Ai-hua;CHI Kun;XU Yong-hua;SUN Jia-ming;LEI Feng-jie;Ginseng Engineering Research Center of Jilin,College of Chinese Medicinal Materials,Jilin Agricultural University;Development Center of Traditional Chinese Medicine and Bioengineering,Changchun University of Traditional Chinese Medicine;
关键词：
Rhizoctonia solani;;Sclerotinia schinseng;;total ginsenosides;;chemotaxis
摘 要：
【Objective】This study investigated the chemotaxis response of Rhizoctonia solani and Sclerotinia schinsengtototal ginsenosides.【Method】Different concentrations of total ginsengsides(200,20,2,0.2mg/L),culture temperatures(10,15,20,25,30℃)and pH values(5,6,7,8)were used to research the chemotaxis response of two pathogens by plate assay.The growth of primary hyphae affected by this chemotaxis was also analyzed.【Result】Rhizoctonia solani exhibited strong positive chemotaxi sagainst total ginsenosides with concentration of 2 mg/L when the culture temperature was 25 ℃ and the pH was 6.Sclerotinia schinsengexhibited strong positive chemotaxisagainst total ginsenosides with concentration of 2mg/L when the culture temperature was 15 ℃ and the pH was 7.The chemotactic migration indexes(CMI)of the two pathogens were 0.136 and 0.149,respectively.【Conclusion】Total ginsenosi desinduced chemotaxis response of Rhizoctonia solani and Sclerotinia schinsengas chemokines.
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