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Position: Home > Articles > Optimization of Methods for Redifferentiation of Pedilanthus tithymaloides var.nanus Hubei Agricultural Sciences 2012,51 (12) 205-206

龙凤竹愈伤组织再分化方法的优化

作  者:
袁云香
单  位:
渭南师范学院化学与生命科学学院
关键词:
龙凤竹[Pedilanthus tithymaloides(L.)Poit var.nanus Dressler];Ca2+;愈伤组织;诱导;再分化
摘  要:
以龙凤竹[Pedilanthus tithymaloides(L.)Poit var.nanus Dressler]茎段为外植体,进行愈伤组织诱导和再分化试验。在已筛选出的培养基中添加不同浓度的Ca2+进行试验,以确定诱导和再分化培养基中的最适的Ca2+浓度。结果表明,适度增加Ca2+浓度可促进龙凤竹愈伤组织的诱导和再分化,但当Ca2+浓度超过15 mmol/L时,促进效果显著降低。
译  名:
Optimization of Methods for Redifferentiation of Pedilanthus tithymaloides var.nanus
作  者:
YUAN Yun-xiang(College of Chemistry and Life Science,Weinan Teachers University,Weinan 714000,Shaanxi,China)
关键词:
Pedilanthus tithymaloides(L.) Poit var.nanus Dressler;Ca2+;callus;induction;redifferentiation
摘  要:
Taking the stems of Pedilanthus tithymaloides(L.) Poit var.nanus Dressler as explants,experiments on callus induction and redifferentiation were conducted.The effect of Ca2+ concentration added to the induction and redifferentiation medium on tissue culture efficiency was studied.The results showed that frequency of induction and redifferentiation could be increased with suitable Ca2+ concentration.However,when the concentration of Ca2+ was higher than 15 mmol/L,the promotion effect was significantly reduced.
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