Position: Home > Articles > Ethanol Reflux Method Extraction and Antioxidant Activities of Total Flavonoids From Lycopodii herba
Northern Horticulture
2016
(6)
116-120
乙醇回流法提取伸筋草总黄酮工艺及其体外抗氧化活性
作 者：
杨申明;范树国;王振吉;杨泽智
单 位：
楚雄师范学院化学与生命科学学院
关键词：
伸筋草;总黄酮;提取工艺;抗氧化性
摘 要：
以伸筋草为试材,在单因素试验的基础上,以总黄酮提取率为指标,采用正交实验L9(34)优化乙醇辅助回流提取总黄酮的最佳工艺条件,并通过总黄酮对羟基自由基和1,1-苯基-2-苦肼基自由基的清除能力来评价其抗氧化性。结果表明:最佳提取工艺参数为乙醇体积分数75%,液料比60∶1mL/g,提取时间150min,提取温度80℃,在此条件下,总黄酮的平均提取率为1.82%;总黄酮质量浓度为0.014 0mg/mL时,对·OH和DPPH·的清除率可分别达72.65%和93.02%,说明总黄酮对·OH和DPPH·有较好的清除能力。
译 名：
Ethanol Reflux Method Extraction and Antioxidant Activities of Total Flavonoids From Lycopodii herba
作 者：
YANG Shenming;FAN Shuguo;WANG Zhenji;YANG Zezhi;Department of Chemistry and Life Science,Chuxiong Normal University;
关键词：
Lycopodii herba;;total flavonoids;;extraction process;;antioxidant properties
摘 要：
The optimal ethanol reflux extraction conditions of total flavonoids fromLycopodii herba were studied.In order to increase the extraction yield of total flavonoids,the ethanol reflux extraction conditions of total flavonoids from Lycopodii herba was optimized by orthogonal test L9(34)based on the single-factor tests.Meanwhile,the antioxidant activity of total flavonoids fromLycopodii herba was evaluated by means of the scavenging capacity of the hydroxyl free radical and the DPPH free radical.The results showed that the optimum extraction parameters were 75%of the volume fraction of ethanol,60∶1mL/g of liquid material ratio,150 minutes of extraction time,80℃ of extraction temperature,and under these conditions,the average extraction rate of total flavonoids was 1.82%.When the concentration of total flavonoids was 0.014 0 mg/mL,the scavenging rates of total flavonoids on ·OH and DPPH· were 72.65% and93.02%,respectively,which implied that the total flavonoids had good ability to scavenge·OH and DPPH·.
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