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Position: Home > Articles > New Method on Streptomycin Residues Assay in Honey by HPLC FOOD SCIENCE 2004,25 (1) 155-158

高效液相色谱法测定蜂产品中链霉素残留量

作  者:
陈晓红;刘小莉;董明盛;陈惠兰
单  位:
南京农业大学食品科技学院农业部重点开放实验室;江苏省出入境检验检疫局
关键词:
链霉素;高效液相色谱;荧光检测;柱后衍生法
摘  要:
采用双试剂柱后衍生法,在C18柱上以0.01mol/L 1-庚烷磺酸钠+乙腈为流动相及荧光检测器(Ex=263nm,Em=447nm)检测蜂蜜中链霉素的残留量,优化了蜂蜜样品的前处理方法和高效液相色谱条件,包括流动相组成和流速及衍生化试剂的处理,同时改善样品前处理过程中淋洗液和提取液的用量,并选用50%三氯乙酸作为沉淀剂,建立了一种更简便、快速、准确性和重现性好的链霉素检测方法。
译  名:
New Method on Streptomycin Residues Assay in Honey by HPLC
作  者:
CHEN Xiao-hong1,LIU Xiao-li1,DONG Ming-sheng1,CHEN Hui-lan2(1.College of Food Science and Technology, Nanjing Agricultural University, Key Laboratory of Ministry Agriculture,Nanjing 210095, China;2.Jiangsu Province Exit/Entry Inspection & Quarantine Bureau, Nanjing 210001, China)
关键词:
streptomycin;HPLC; fluorescence;post-column derivatization
摘  要:
HPLC with post-column derivatisation was used to detect Streptomycin residues in honey, with 0.01mol/l sodium1-Heptanesulfonate and acetonitrile(65+35) as mobile phase and fluorescence detection (Ex=263nm,Em=447nm). Detectionconditions and parameters including the proportion and speed of mobile phase, arrangement of deriving agents, dosage of solutionand elution and usage of sedimentary agent were optimized. A new method with simplicity, repeatability and accuracy wasestablished in this paper.

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