译 名：
Detection of Genetically Modified Soybean in Feedstuff by Multiplex PCR
作 者：
YU Zhong-na~(1,2) MIAO Xiang-yang~(1,2) SHAN Hu~(1,2) LI Jian-xia~(1,2) 1.Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,State Key Laboratory of Animal Nutrition,Beijing,100094,China; 2.Laiyang Agricultural College,Qingdao,Shandong,266109,China
关键词：
bean meal feed;;transgenic components;;multiplex PCR
摘 要：
Endogenous gene Lectin,exogenous gene CaMV35 S promoter,NOS terminator and 35 S-CTP4 were chosen for establishing a multiplex polymerase chain reaction method.The PCR conditions for the multiplex PCR analysis were optimized according to the primer concentration and annealing temperature. The results showed that the high efficiency PCR system can differentially,conveniently,effectively and coinstantaneously examine the endogenous gene Lectin and the transgenic components.This method is highly improved the examining efficiency and can be used for the detection of transgenic components in ge- netically modified bean meal.
