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检测猪流行性腹泻病毒的R-PCR方法的建立

作  者:
倪艳秀;何孔旺;俞正玉;郭蓉利;吕立新;陈兆霞
单  位:
江苏省农业科学院农业部畜禽疫病诊断重点开放实验室
关键词:
猪流行性腹泻病毒;RT-PCR;猪轮状病毒;猪传染性胃肠炎病毒
摘  要:
根据猪流行性腹泻病毒 (PEDV)的N基因自行设计和合成了一对可扩增长度为 641bp目的片段的引物 ,成功地建立了检测的猪流行性腹泻病毒的RT PCR方法。对猪轮状病毒 (PRV)、猪传染性胃肠炎病毒 (TGEV)的RT PCR检测结果均呈阴性。对PEDV JS株的RT PCR产物的序列分析表明 ,与CV777株的同源性为 97 3 %。
译  名:
Detection of porcine epidemic diarrhea virus by RT-PCR
作  者:
NI Yan-xiu,HE Kong-wang,YU Zheng-yu, GUO Rong-li,LU Li-xing,CHEN Zhao-xia (Key Lab of Animal & Poultry Diseases Diagnosis,Ministry of Agric, Jiangsu Academy of Agric Sciences,Nanjing 210014,China)
关键词:
porcine epidemic diarrhea virus;RT-PCR;transmissible gastroenteritis virus;porcine rotavirus
摘  要:
A PCR assay for rapid and sensitive detection of porcine epidemic diarrhea virus was developed.The PCR primers based on the N gene of porcine epidemic diarrhea virus could extend a 641 bp PCR product.Using the same pairs,no PCR product was detected from porcine rotavirus and transmissible gastroenteritis virus.The sequence homology of RT-PCR products of PEDV-JS strain and CV777 strain was 97.3%.

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