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当前位置: 首页 > 文章 > 盾壳霉抗菌核净菌株的诱导及其特性初步研究 农药学学报 2005,7 (3) 233-236
Position: Home > Articles > Primary Study on Induction and Characteristics of Dimethachlon-resistant Strains of Coniothyrium minitans Chinese Journal of Pesticide Science 2005,7 (3) 233-236

盾壳霉抗菌核净菌株的诱导及其特性初步研究

作  者：
赵丽娟;张永杰;刘慧平;韩巨才;高俊明
单  位：
山西农业大学农学院
关键词：
盾壳霉;菌核净;突变菌株;诱导;特性
摘  要：
为筛选可与菌核净配合防治作物菌核病的盾壳霉菌株,进行了紫外线辐射和药剂驯化试验,分别得到4个和2个盾壳霉抗菌核净突变菌株,并研究了其生物学特性。结果表明:除菌株D-1外,其余突变菌株生长速率(菌落直径57.00~62.75 mm,生物量6.38~8.63 mg/mL)均大于亲本菌株(菌落直径54.00 mm,生物量5.91 mg/mL);大部分突变菌株产孢量(对数值7.33~8.16)小于亲本菌株(8.40);菌核净对突变菌株的EC50值是亲本菌株的2.23~191.84倍;突变菌株D-3、U-6有微弱的几丁质酶活性,且除U-4外的突变菌株葡聚糖酶活性增强(亲本菌株8.96 U,突变菌株9.18~17.61 U)。综合分析,U-6菌株既有较高的葡聚糖酶活性又有几丁质酶活性,并能保持较快的生长速率,是值得开发的优良菌株。
译  名：
Primary Study on Induction and Characteristics of Dimethachlon-resistant Strains of Coniothyrium minitans
作  者：
ZHAO Li-juan, ZHANG Yong-jie, LIU Hui-ping~*, HAN Ju-cai, GAO Jun-ming(College of Agriculture,Shanxi Agricultural University,Taigu Shanxi 030801,China)
关键词：
Coniothrium minitans; dimethachion; mutants; induction; characteristics
摘  要：
Six dimethachlon-resistant mutants of Coniothyrium minitans were obtained by ultraviolet (irradiation )and chemical-taming in order to acquire strains being able to integrate with dimethachlon (against sclerotinia) rot diseases. The results of characteristics of mutant strains showed that growth rates of mutant strains (colony diameter 57.00~62.75 mm, biomass 6.38~8.63 mg/mL) except D-1 were larger than parent strain (colony diameter 54.00 mm, biomass 5.91 mg/mL); spore yields of most (mutant) strains (logarithm of spore yields 7.33~8.16) were less than parent strain (8.40); EC_(50) (values) of mutant strains were 2.23~191.84 folds higher than parent strain; only mutant strains D-3 and U-6 had slight chitinase activities and glucanase activities of mutant strains(9.18~17.61 U) but U-4 (enhanced) compared with parent strain(8.96 U).In general, mutant strain U-6 had not only higher (chitinase )activities but also possessed chitinase activities. Besides, U-6 maintained faster growth rate, so it is a strain worthy of further development.

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