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Position: Home > Articles > Effects of Sodium Nitro-prusside on the Granulosa Apoptosis Rate of Porcine Preantral Follicles China Animal Husbandry & Veterinary Medicine 2010,37 (12) 124-127

一氧化氮对猪腔前卵泡颗粒细胞凋亡的影响

作  者:
权青;陶勇;章孝荣;汪金菊;姚桂东
单  位:
安徽农业大学经济技术学院;安徽农业大学动物科技学院;合肥市妇幼保健医院生殖中心
关键词:
猪;腔前卵泡;颗粒细胞凋亡
摘  要:
本试验旨在研究在培养液中添加不同浓度的NO供体硝普钠(sodium nitro-prusside,SNP)(0、0.001、0.01、0.1、1.0 mmol/L)处理,研究培养4 d后的腔前卵泡颗粒细胞凋亡情况。结果表明,1.0 mmol/L SNP组凋亡率显著高于其他各组(P<0.05),而0.001 mmol/L SNP组凋亡率显著低于其他各组(P<0.05)。说明高浓度的SNP对猪腔前卵泡颗粒细胞凋亡起促进作用,低浓度的SNP起抑制作用。
译  名:
Effects of Sodium Nitro-prusside on the Granulosa Apoptosis Rate of Porcine Preantral Follicles
作  者:
QUAN Qing1,2,TAO Yong2,ZHANG Xiao-rong2,WANG Jin-ju2,YAO Gui-dong3(1.College of Economy and Technology,Anhui Agricultural University,Hefei 230036,China;2.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China;3.Center for Reproductive Medicine,Hefei Maternity and Children's Hospital,Hefei 230001,China)
关键词:
porcine;preantral follicles;granulose apoptosis
摘  要:
In order to understand the effects of nitric oxide(NO) on granulose apoptosis of porcine preantral follicles at four days of the culture,we treated the follicles with different concentrations of sodium nitro-prusside(0,0.001,0.01,0.1 and 1.0 mmol/L SNP),a nitric oxide donor.The results indicated that 1.0 mmol/L SNP was significantly higher than other treatments(P<0.05),0.001 mmol/L SNP was significantly lower than other treatments(P<0.05).In conclusion,high concentrations of SNP in culture medium can significantly stimulate follicles granulose apoptosis,low concentrations of SNP inhibit follicles granulose apoptosis.

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