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当前位置: 首页 > 文章 > 不同品种荞麦耐铝性的FTIR鉴别 浙江大学学报(农业与生命科学版) 2010,36 (6) 683-690
Position: Home > Articles > Endurance determination of different buckwheat cultivars to aluminum by FTIR spectrum Journal of Zhejiang University (Agriculture and Life Sciences) 2010,36 (6) 683-690

不同品种荞麦耐铝性的FTIR鉴别

作  者：
张亚楠;王赢;陈奇;黄庶识;李昆志
单  位：
昆明理工大学
关键词：
傅里叶变换红外光谱法;荞麦;耐铝性
摘  要：
通过傅里叶变换红外光谱法(FTIR)结合常规的根伸长实验法对2个不同耐铝性的荞麦品种(春红1号和春红4号)进行分析鉴定.通过比较春红1号和春红4号在不同铝浓度处理下根、茎和叶的红外光谱图特征峰3353、2923、1734、1643、1453、1367、1250和1055cm-1发现:春红1号的特征峰值高于春红4号;在铝胁迫下,春红1号和春红4号的根、茎和叶的FTIR特征峰值随着铝浓度的升高而增加,当铝浓度增至50μmol?L-1时,春红1号达到最大值;而春红4号在20μmol?L-1时达到最大值;此外,在铝胁迫下,春红4号根、茎和叶的FTIR特征峰值的变化幅度比春红1号大,说明春红4号物质代谢受到的影响较大,表现出对铝敏感.根伸长实验结果表明:当铝浓度为20μmol?L-1时,促进春红1号根的生长,根伸长量是对照的140%,达到最大值,而春红4号的根生长受到很大的抑制,伸长量只有对照的80%.以上研究结果表明,春红1号比春红4号的耐铝能力强,FTIR法可以用作鉴别不同品种荞麦耐铝性的一种新的、快速、准确的检验方法.
译  名：
Endurance determination of different buckwheat cultivars to aluminum by FTIR spectrum
作  者：
ZHANG Ya-nan1,WANG Ying1,CHEN Qi1,HUANG Shu-shi1,2,LI Kun-zhi1(1.Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650224,China;2.Lab of Biophysics,Guangxi Academy of Sciences,Nanning 530007,China)
关键词：
Fourier transform infrared(FTIR)spectroscopy;buckwheat;Al tolerance
摘  要：
The Al-tolerance of two different varieties(Chun-Hong 1,Chun-Hong 4)of buckwheat was analyzed and identified by Fourier transform infrared(FTIR)spectroscopy and conventional experimental method of root elongation.By comparison of root,stem and leaf characteristics of the infrared spectra peaks 3 353,2 923,1 734,1 643,1 453,1 367,1 250 and 1 055 cm-1 between the two varieties under the different Al concentrations,most of the characteristic peaks in Chun-Hong 1 were higher than that in Chun-Hong 4.Characteristics of the FTIR peak for the root,stem and leaf in Chun-Hong 1 and Chun-Hong 4 were increased with increase of Al concentrations.FTIR peak of Chun-Hong 1 reached the maximum when the Al concentration increased to 50 μmol·L-1,while that of Chun-Hong 4 reached the maximum at the Al concentration of 20 μmol·L-1.Besides,the results showed that the FTIR spectra of Chun-Hong 4 varied much greater in roots,stems and leaves than aluminum-resistant cultivar Chun-Hong 1 under different Al concentrations,implying that Chun-Hong 4 metabolism was evidently affected by Al.The results of root elongation experiment showed that the root growth of Chun-Hong 1 was promoted and the amount of root elongation reached the maximum of 140% compared with the control,while that of Chun-Hong 4 was greatly inhibited and its elongation was only 80% of the control with the treatment of 20 μmol·L-1 Al.These findings demonstrate that Al endurance of Chun-Hong 1 is stronger than Chun-Hong 4,and FTIR spectra can be developed as a new,rapid and accurate method for the endurance identification of different buckwheat cultivars to Al.

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