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Position: Home > Articles > Transformation with P1 Gene from Foot and Mouth Disease Virus into Maize Journal of Maize Sciences 2004,12 (3) 22-25

口蹄疫病毒结构蛋白P1基因转化玉米的初步研究

作  者:
余云舟;王罡;金宁一;季静;王萍
单  位:
解放军军需大学植物基因工程研究中心
关键词:
玉米;口蹄疫病毒;P1基因;转基因;再生植株
摘  要:
实验在构建口蹄疫病毒(FootandMouthDiseaseVirus,FMDV)结构蛋白P1基因植物双元表达载体(pBI131SP1、pBI21P1和pBI121AP1)的基础上,以玉米自交系8902、340、4112的Ⅱ型胚性愈伤组织为受体,用农杆菌介导法和基因枪轰击法转化玉米,获得31株抗性再生植株。GUS染色证明外源目的基因在玉米细胞和组织中得到了表达。PCR检测、Southernblotting鉴定证实目的基因P1已整合到再生植株的基因组中,共获得了13株转基因玉米植株,首次进行了FMDV抗原基因转化玉米的研究。
译  名:
Transformation with P1 Gene from Foot and Mouth Disease Virus into Maize
作  者:
YU Yun-zhou, WANG Gang, JIN Ning-yi, JI Jing, WANG Ping(Research Center for Plant Genetic Engineering, Quartermaster University of PLA, Changchun 130062, China)
关键词:
Maize: FMDV: P1 gene: Transformation: Regenerated plant
摘  要:
FMDV-P1, neutralizing site determined, which induces protective antibody and immune response of animal, is subjective of gene engineering vaccine research. Plant binary expression vectors pBI131SP1, pBI121P1 and pBI121AP1 were constructed with P1 gene from FMDV. Type Ⅱ calli were initiated from immature embryos of maize inbred line 8902, 340 and 4112, and were transformed with FMDV-P1 via Agrobacterium tumefaciens(LBA4404 and EHA105) and particle bombardment. 31 kanamycin-resistant transformants have been obtained in this experiment. GUS assay indicated that foreign gene had expressed in maize cell and calli. It was demonstrated that P1 gene had been inserted into maize genome after PCR and Southern blotting analysis of regenerated plants. 13 transgenic maize plants have been obtained. To our knowledge, this is the first report that FMDV-P1 gene was transformed into maize.

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