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Position: Home > Articles > Opioid Antagonist Activities of Enzymatic Hydrolysates from Rice Bran Protein FOOD SCIENCE 2005,26 (6) 141-145

米糠蛋白酶解物类阿片拮抗活性的研究(英文)

作  者:
陈季旺;孙庆杰;姚惠源;夏文水
单  位:
湖南金健米业股份有限公司;江南大学食品学院;武汉工业学院食品学院
关键词:
米糠蛋白;类阿片拮抗活性;离体豚鼠回肠(GPI)检定法
摘  要:
采用水解度(DH)对酶解米糠蛋白进行了研究,比较六种蛋白酶对米糠可溶性蛋白的水解作用,结果发现胰蛋白酶对米糠可溶性蛋白的水解作用最强,它的最适作用条件为:pH8.0,温度为37℃,[E]/[S]为12.5usp-u/kg。采用离体豚鼠回肠(GPI)检定法测定上述酶解物的类阿片拮抗活性,结果发现胰蛋白酶水解产物具有明显的类阿片拮抗活性,DH为11.9%时,其水解产物(样品A)的类阿片拮抗活性最高。体积排阻高效液相色谱测定它的相对分子质量分布范围在125~24233Da之间。
译  名:
Opioid Antagonist Activities of Enzymatic Hydrolysates from Rice Bran Protein
作  者:
CHEN Ji-wang1,Sun Qing-jie2,YAO Hui-yuan3 (1.Food School of Wuhan Polytechnic Unversity, Wuhan 430023, China; 2.Hunan Jinjian Cereals Industry Co. Ltd, Changde 415001, China; 3.School of Food Science, Southern Yangtz Unversity,Wuxi 214036, China)
关键词:
rice bran protein;opioid antagonist activity;guinea pig ileum-contracting(GPI)assays
摘  要:
The enzymatic hydrolysis of rice bran protein was studied with degree of hydrolysis. Trypsin was found to be the most effective protease and its optimum conditions were pH8, ratio of enzyme to substrate 1:100 and temperature of 37℃. Resulting hydrolysates were tested for their opioid antagonist activities based on guinea pig ileum-contracting assayss. The hydrolysate produced at 11.9% of degree of hydrolysis showed the highest opioid antagonist activity. High performance size exclusion chromatography results indicated that its molecular weight distribution mainly ranged from 125~24233Da.

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