Cloning and Expression Analysis of TaABCF in Wheat Near Isogenic Line TcLr19

YUAN Ying;HU Yaya;LI Jianyuan;YANG Wenxiang;LIU Daqun;Department of Plant Pathology,Agricultural University of Hebei/Biological Control Center of Plant Diseases and Plant Pests of Hebei Province/National Engineering Research Center for Agriculture in Northern Mountainous Areas;Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences;

TaABCF;;Gene clone;;Wheat leaf rust;;Expression analysis;;Signal pathway

In order to discover new genes against wheat leaf rust,this study was carried out to clone resistance related genes TaABCFby RACE(rapid amplification of cDNA ends)technique from TcLr19 infected by a virulent Puccinia triticina,and its expression analysis was analysed.The full length cDNA and genomic DNA of the TaABCFgene are 1 885 bp and 3 100 bp,respectively.And it contains 4 introns and 5 exons,and has two nucleotide binding domains and each contains Walker A,Walker B and Walker C.The phylogenetic analyses showed that TaABCF has the closest relationship with EMS53714.1 gene from Triticum urartu Thum.ex Gandil.Expression analysis with real-time fluorescent quantitative PCR showed that the expression of TaABCFwas improved in the incompatible interaction between TcLr19 and P.triticina FHPL in the early stage,while inhibited in the compatible interaction between Thatcher and FHPL latterly.The expression of TaABCF were earlier when treated with ABA and MeJA than that of SA,which showed that TaABCFgene is involved in the resistance of wheat leaf rust and it responds to ABA and MeJA earlier than SA.