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Position: Home > Articles > Cloning and Sequence Analysis of Bovine Interferon-gamma Gene Animal Husbandry and Feed Science 2009,30 (3) 18-19+21

牛γ-干扰素基因的克隆及序列分析

作  者:
Dongyan Sh
单  位:
College of Animal Science and Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, Chin
关键词:
gene;amplified;bovine;rt;bovifn;interferon-gamm
摘  要:
根据GenBank中已公布的牛γ-干扰素(BovIFN-γ)基因序列设计引物,应用一步法逆转录—聚合酶链式反应(RT-PCR)技术,以从牛外周血淋巴细胞提取的总RNA为模板,扩增出γ-干扰素成熟肽基因片断,并对其进行了克隆、测序及序列分析。结果表明,试验所设计引物可以成功用于扩增牛γ-干扰素成熟肽基因片段,所扩增序列与GenBank中已有序列同源性达99.77%。
译  名:
Cloning and Sequence Analysis of Bovine Interferon-gamma Gene
作  者:
WU Yu-mei,HAO Yong-qing,ZHANG Ai-rong,SHI Dong-yan(College of Animal Science and Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)
关键词:
BovIFN-γ;RT-PCR method;cloning;sequence analysis
摘  要:
Based on the published nucleotide sequence of bovine interferon-gamma(BovIFN-γ)gene,a pair of RT-PCR primers were designed and synthesized.The mature peptide fragments of γ-interferon gene was amplified using reverse transcription polymerase chain reaction(RT-PCR)from total RNA of bovine peripheral blood lymphocytes,and then carried out cloning,sequencing and sequence analysis.The results showed that the mature peptide of BovIFN-γ gene can be successfully amplified by primers designed.Additionally,the homology between the amplified gene fragment sequence and the relevant sequence in GenBank can reach up to 99.77%。

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