中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 恩拉霉素人工抗原的合成与鉴定 湖北农业科学 2019 (7) 92-95+156
Position: Home > Articles > Synthesis and identification of enramycin artificial antigen Hubei Agricultural Sciences 2019 (7) 92-95+156

恩拉霉素人工抗原的合成与鉴定

作  者：
叶健强;余华;康润敏;严玉宝;谢晶;胡娟;廖党金;周岷江;肖璐;崔鹏博;曹冶;叶勇刚;于吉锋;李兴玉;林毅;潘梦;魏甬;戴卓建
关键词：
恩拉霉素;抗原合成;戊二醛法
摘  要：
建立酶联免疫方法以检测食品中恩拉霉素的残留,采用戊二醛法,偶联半抗原恩拉霉素(Er)和载体牛血清白蛋白(BSA)或卵清白蛋白(OVA),制备人工免疫抗原和人工包被抗原,分别为Er-BSA和Er-OVA。经紫外光谱鉴定,在紫外270～280 nm波长处得到Er-BSA和Er-OVA特征性偶联吸收峰。试验结果表明人工合成的2个人工抗原均偶联成功。人工免疫抗原和人工包被抗原的偶联比均达到26∶1,为进一步制备抗血清和酶联检测方法的建立奠定了基础。
译  名：
Synthesis and identification of enramycin artificial antigen
作  者：
YE Jian-qiang;YU Hua;KANG Run-min;YAN Yu-bao;XIE Jing;HU Juan;LIAO Dang-jin;ZHOU Min-jiang;XIAO Lu;CUI Peng-bo;CAO Ye;YE Yong-gang;YU Ji-feng;LI Xing-yu;LIN Yi;PAN Meng;WEI Yong;DAI Zhuo-jian;Sichuan Animal Science Academy;Key Laboratory of Animal Genetics and Breeding of Sichuan Province;Sichuan Entry-Exit Inspection and Quarantine Bureau;
关键词：
Enramycin;;synthesis of antigen;;glutaraldehyde method
摘  要：
In order to establish an enzyme-linked immunosorbent assay(ELISA) for examing residual Enramycin(Er) in food.The antibody with high titer and specificity must be firstly prepared. So it was necessary for antibody production to conjugate the hapten(Er) with a carrier protein to synthesize immunogent(antigen). Enramycin was coupled with carrier protein bovine serum albumin(BSA) to obtain immunogent(Er-BSA) by glutaraldehyde(GA) method and with ovalnumin(OVA) to obtain coating antigen(Er-OVA) by GA respectively. The successful linkage of Er-BSA and Er-OVA were identified by UV spectrophotometry. The UV spectral characterization demonstrated that Er-BSA and Er-OVA were successfully synthesized at 270～280 nm. The coupling ratios between Er and BSA or OVA were determined to be 26∶1, respectively.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46