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当前位置: 首页 > 文章 > 我国犬埃立克体病病原的分离与鉴定 Ⅰ.病原16S rRNA基因序列测定与分析 中国兽医学报 1999 (5) 467-470
Position: Home > Articles > Isolation and Identification of the Causative Agent of Canine Ehrlichiosis Found in China Ⅰ.Sequencing and Analyzing of 16S rRNA Gene Chinese Journal of Veterinary Science 1999 (5) 467-470

我国犬埃立克体病病原的分离与鉴定 Ⅰ.病原16S rRNA基因序列测定与分析

作  者：
潘华;孙洋;佟世德;马玉海;温博海;陈香蕊
单  位：
第三军医大学;广州军区军事医学研究所;军事医学科学院微生物流行病研究所
关键词：
埃立克体;分离;鉴定;基因序列;犬
摘  要：
用实验动物接种法，分离保存我国某养犬基地犬埃立克体病病原。以此病原对易感犬接毒，接毒犬发病后采集急性期全血提取基因组，对病原 １６ Ｓｒ Ｒ Ｎ Ａ 基因进行扩增、克隆测序。结果，病原被保存在病犬血细胞内，并能成功地使健康犬感染发病；检测到的 ２ 种致犬埃立克体病病原 １６ Ｓ ｒ Ｒ Ｎ Ａ 基因序列与国外已发现的相应病原基因序列有一定差异，显示其为不同菌株，分别命名为埃立克体 Ｇｚｈ ９８１ 株和 Ｇｚｈ９８２ 株。进一步分析表明，导致我国某养犬基地犬埃立克体病的病原为扁平埃立克体和犬埃立克体。
译  名：
Isolation and Identification of the Causative Agent of Canine Ehrlichiosis Found in China Ⅰ.Sequencing and Analyzing of 16S rRNA Gene
作  者：
PAN Hua 1, SUN Yang 1, TONG Shi de 1, MA Yu hai 1, WEN Bo hai 2, CHENG Xiang rui 3 (1. Guangzhou Military Medical Institute, Guangzhou 510507, China; 2. The Third Military Medical University, China; 3. Academy of Military Medical Sciences,
关键词：
Ehrlichia; isolation; identification; gene sequence; canine
摘  要：
The causative agent of canine ehrlichiosis had been isolated by animal infection experiment. Then the causative agent was inoculated Beagle pup. Blood was drawn when canine got in fecer, and the genome was extracted. 16S rRNA gene of the agent was amplified, cloned and sequenced. The pathogens were stored in leukocyte including platelet at -80℃, and could infect Beagle pup again. Two different sequences of 16S rRNA gene were amplified and sequenced. There was a discrepancy in the base pair between the 16S rRNA gene of the pathogens and that of Ehrlichia which had been reported respectively. The results showed that the causative agents of canine ehrlichiosis found in China were two species Ehrlichia strain Gzh981 and strain Gzh982. They were regarded as E.platys and E.canis.

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