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当前位置: 首页 > 文章 > 银白杨与白榆杂交后代有性生殖能力的研究 西北林学院学报 2010,25 (2) 56-60
Position: Home > Articles > Sexual Reproduction Capability of Populus alba L. × Ulmus pumila L. Journal of Northwest Forestry University 2010,25 (2) 56-60

银白杨与白榆杂交后代有性生殖能力的研究

作  者：
杨成超;王胜东;杨志岩;李晓鹏;张研;赵继梅
单  位：
辽宁省杨树研究所
关键词：
有性生殖;花粉管畸形;银白杨;白榆
摘  要：
从雌、雄两方面来研究科间杂种银榆杨(银白杨×(白榆+新疆杨失活花粉))的有性生殖能力。(1)以新疆杨、辽宁杨和辽胡3号杨为对照,采用花序数量、花粉量及花粉生命力3个指标评价银榆杨的雄性生殖能力,测定花粉生命力采用醋酸洋红染色、培养基培养、杂交试验3种方法。结果表明,在参试品种中,银榆杨雄花序数量最大,花粉量最小,花粉含水率最低。银榆杨花粉在培养基上萌发时花粉管出现扭曲、分叉等畸形现象,占萌发花粉的30%。以银榆杨为父本进行杂交育种可能出现杂交不亲和现象。(2)以银白杨为对照,用杂交法研究银榆杨雌性孕性。结果表明,银榆杨雌性可育,但与不同派品种的亲和力不同。最后,对银榆杨花粉管在培养基上萌发存在畸形的原因进行了讨论,对银榆杨应用于杂交育种提出建议。
译  名：
Sexual Reproduction Capability of Populus alba L. × Ulmus pumila L.
作  者：
YANG Cheng-chao1,2,WANG Sheng-dong1,YANG Zhi-yan1,LI Xiao-peng1,ZHANG Yan1,ZHAO Ji-mei1(1.Liaoning Provincial Institute of Poplar,Gaizhou,Liaoning 115200,China;2.Research Institute of Forestry,CAF,Beijing 100091,China)
关键词：
sexual reproduction;pollen tube abnormality;Populus alba L.;Ulmus pumila L.
摘  要：
An out-crossing experiment bettween Populus alba L.×(Ulmus pumila L.+DEAD P.alba L.var.pyramidalis) was conducted.By using techniques of pollen inducement and puerile ovary culture,the out-crossing barrier was conquered,and hybrid seedling of P.alba × U.pumila were successfully produced.The hybrids of P.alba ×(U.pumila L.+DEAD P.alba L.var.pyramidalis) were named P.× alba L.'yinyu'.Sterility usual occurred in hybridization of distant taxa.Sexual reproduction of interfamilial hybrids is concerned widely.Sexual reproduction of P.× alba L.'yinyu' was studied in male and female from the following aspects:(1) The number of inflorescences,weight of pollen and vitality of pollen were recorded to evaluate sexual reproduction.P.× Liaonignica sp.Nov.,P.alba L.var.pyramidalis and(P.simonii × P.euphratica) × P.nigra L.var.italica(Moench.) were used as controls.Pollen viability was detected by aceto carmine,culture medium and mating.The results indicated that the number of inflorescences of P.× alba L.'yinyu' was the greatest,while the weight and vitality of pollen were the lowest among all tested samples.There were about 30% pollen tubes that were tortuose and forficate in culture medium.Colligating the results of dye and crossing,cross incompatibility was possible when P.× alba L.'yinyu' was the male parent;(2) an pollination test of P.× alba L.'yinyu' was conducted and P.× alba L.was used as a control.Female P.× alba L.'yinyu' was pollinated,but showing difference affinities with different male parents.At last,the causes for pollen tube abnormality of P.× alba L.'yinyu' were discussed.Some recommendations were also made on using P.× alba L.'yinyu' as a parent.

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