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贮存条件对阳春砂花粉活力的影响

作  者:
于安民;杨锦芬;詹若挺;陈蔚文
单  位:
广州中医药大学中药资源科学与工程研究中心
关键词:
阳春砂;花粉活力;贮存条件;离体萌发法;MTT染色法
摘  要:
以种植于广州中医药大学的阳春砂为试材,将收集的花粉贮存在不同的温度和湿度条件下,分别在贮存0、3、6、9、12、24、48h后,用离体花粉萌发法和噻唑蓝(MTT)染色法测定花粉活力,以期探讨不同贮存条件对阳春砂花粉活力的影响.结果表明:适当的高温和高湿度条件能延长阳春砂花粉的贮存寿命.离体花粉萌发法比MTT染色法能更真实的反映阳春砂花粉活力;27℃+高湿度的条件最适合阳春砂离体花粉的短期(≤9h)贮存,这与阳春砂喜高温高湿的生长习性相符.
单  位:
Research Center of Chinese Herbal Resource Science and Engineering,Guangzhou University of Traditional Chinese Medicine,Guangzhou,Guangdong 510006%Key Laboratory of Chinese Medicine Resource from Lingnan(Guangzhou University of Chinese Medicine
关键词:
A.villosum%pollen viability%preservation condition%pollen germination in vitro%MTT staining method
摘  要:
Taking Amomum villosum Lour.that cultivated in Guangzhou University of Chinese Medicine as material,the effect of different preservation conditions on the pollen viability of Amomum villosum Lour.were studied.The collected pollen were kept in different conditions for 0,3,6,9,12,24 and 48 hours respectively,then the pollen viability were determined using MTT staining method and in vitro germination method.The results showed the moderately high temperature and high humidity prolonged the life of pollen from A.villosum.In vitro germination method was appropriate to reveal the pollen viability.The optimum short-term storage(≤9 h)condition for pollen of A.villosum was 27℃ high humidity,which was consistent with the growth habit of high temperature and humidity.

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