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当前位置: 首页 > 文章 > 免疫酶标记诊断急性猪弓形体病试验报告 畜牧兽医学报 1981 (1) 61-65
Position: Home > Articles > THE IMMUNOENZYMATIC TECHNIQUE FOR RAPID DIAGNOSIS OF ACUTE CASES OF SWINE TOXOPLAMOSIS Chinese Journal of Animal and Veterinary Sciences 1981 (1) 61-65

免疫酶标记诊断急性猪弓形体病试验报告

作  者：
林继煌;范文明;江学余;仇家宏;周元根;计浩
摘  要：
本文是介绍以辣根过氧化物酶标记弓形体免疫血清球蛋白检查弓形体病抗原的新方法。应用本方法活体或死后检查了人工感染的弓形体病猪,活体检查检出率为60-80%;死后检查检出率为100%。经与一般姬姆萨染色法比较,证实免疫酶技术有较高的敏感性和特异性,而且方法简便,设备简单,可在野外应用。本方法可作为猪弓形体病发病初期的快速诊断方法。
译  名：
THE IMMUNOENZYMATIC TECHNIQUE FOR RAPID DIAGNOSIS OF ACUTE CASES OF SWINE TOXOPLAMOSIS
作  者：
Lin Gixuang Fan Wenming Giang Xyoy Chou Giaxung Zhou Yangan Gi Xao Research Institute of Animal Husbandry and Veterinary Medicine,Jiangsu Academy of Agricultural Science,Nanjing
摘  要：
In one experiment 11 Pigs were inoculated intravenously with 14-25 million trophozoites of Toxop- lasma gondii.In this group of experimently infected pigs toxoplasmas were detected in 36 principal organs of 6 acute cases of swine toxoplasmosis by this technique,the positive rate being 94.4%; whereas during life of these 11 pigs when the superficial inguinal lymph nodes were examined for the parasites,the positive rate was 100%. In another experiment 25 pigs were inoculated intravenously with one million trophozoites of Toxo- plasma gondii,and the positive rate of using this technique to detect the parasites in the superficial inguinal lymph nodes of the living pigs was 60%～80%. When comparing with the results obtained by the ordinary Giemsa's staining method the immun- oenzymatic technique was found to be much more sensitive and specific,and the amount of various reagents used was very small,their qualities were rather stable,and the treated specimens could be examined with an ordinary light microscope without any nonspecific interference.On this account, it is considered that the immunoenzymatic technique is easier to be used in an ordinary laboratory than the Giemsa's staining and could provide a means for the rapid diagnosis of acute cases of swine toxoplasmosis.

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