当前位置: 首页 > 文章 > 苦豆草生物碱对松材线虫的抑制作用 南京林业大学学报(自然科学版) 2006,30 (6) 129-131
Position: Home > Articles > Inhibitory Effects of Alkaloids of Sophora alopecuroids on Pine Wood Nematode(Bursaphelenchus xylophilus) Journal of Nanjing Forestry University(Natural Sciences Edition) 2006,30 (6) 129-131

苦豆草生物碱对松材线虫的抑制作用

作  者:
赵博光;赵贞伟;王华光
单  位:
南京林业大学森林资源与环境学院
关键词:
苦豆草;生物碱;苦豆碱;松材线虫
摘  要:
分别用槐果碱、苦参碱、槐胺碱、苦豆碱、野靛碱、N-甲基野靛碱、莱曼碱和总碱配制成生物碱浓度为1×10-4g/mL的PDA培养基,并在培养基上接种灰葡萄孢菌后再接种松材线虫,观察、记录松材线虫繁殖的情况。结果表明,苦豆碱、野靛碱和总碱对线虫的抑制繁殖作用都较强,其中,苦豆碱能完全抑制线虫的繁殖,且能引起线虫的死亡。对测定的生物碱的分子结构及对松材线虫的活性的分析进一步验证了关于双稠哌啶类生物碱对松材线虫的活性与其分子结构中的官能团对之间关系的假说。
译  名:
Inhibitory Effects of Alkaloids of Sophora alopecuroids on Pine Wood Nematode(Bursaphelenchus xylophilus)
作  者:
ZHAO Bo-guang,ZHAO Zhen-wei,WANG Hua-guang(College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037,China)
关键词:
Shophora alopecuroides;Alkaloid;Aloperine;Bursaphelenchus xylophilus
摘  要:
Sophocapine,matrine,oxymatrine,sophramine,aloperine,cytisine,N-methylcytisine,lehmannine and the total alkaloids of the plant were respectively used to add in the PDA medium,the alkaloid concentration in which was 1×10~(-4)g/mL.Then Botrytis ceneria was cultured in the media,on which the pine wood nematodes(Bursaphelenchus xylophilus) was cultured.The reproduction of the nematode on the medium was observed and recorded.The results indicated that high inhibitory effects were observed in the treatments of aloperine,cytosine and total alkaloids,among which aloperine could not only completely inhibit reproduction,but also cause mortality of the nematode.The analysis of the activity of the tested alkaloids on the pine wood nematodes and their molecular structures demonstrated the hypothesis on relationship between activity of the quiniolizidine alkaloids on pine wood nematode and their functional group pairs(FGP) in the molecular.

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