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当前位置: 首页 > 文章 > 非洲马瘟VP7蛋白多克隆抗体的制备及IgM捕获ELISA检测方法的建立 中国动物检疫 2014,31 (5) 70-73
Position: Home > Articles > Preparation of Polyclonal Antibody Against Protein VP7 of African Horse Sickness Virus and Development of IgM capture Enzyme-linked Immunosorbent Assay for Detecting African Horse Sickness China Animal Health Inspection 2014,31 (5) 70-73

非洲马瘟VP7蛋白多克隆抗体的制备及IgM捕获ELISA检测方法的建立

作  者：
郑小龙;朱来华;王群;艾军;邓明俊;肖西志;梁成珠;姜帆;于业锋
单  位：
云南出入境检验检疫局;山东出入境检验检疫局
关键词：
非洲马瘟;IgM捕获ELISA(MAC-ELISA);VP7蛋白
摘  要：
为建立早期快速检测非洲马瘟抗体的IgM捕获ELISA(MAC-ELISA)方法,本试验利用大肠杆菌表达出具有良好抗原性的VP7蛋白,并制备该蛋白的多克隆抗体,通过优化抗原浓度、血清稀释度及酶标抗体稀释度等,建立了检测非洲马瘟的MAC-ELISA方法,并进行了初步应用。结果表明,利用重组表达的VP7蛋白成功建立了检测非洲马瘟的MAC-ELISA方法。
译  名：
Preparation of Polyclonal Antibody Against Protein VP7 of African Horse Sickness Virus and Development of IgM capture Enzyme-linked Immunosorbent Assay for Detecting African Horse Sickness
作  者：
Zheng Xiaolong;Zhu Laihua;Wang Qun;Ai Jun;Deng Mingjun;Xiao Xizhi;Liang Chengzhu;Jiang Fan;Yu Yefeng;Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China;Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China;
关键词：
African horse sickness;;IgM capture enzyme-linked immunosorbent assay(MAC-ELISA);;Protein VP7
摘  要：
The study was aimed to develop an IgM capture enzyme-linked immunosorbent assay for detecting African horse sickness(AHS). The AHSV VP7 protein with good immunogenicity was expressed in BL21 and polyclonal antibody against protein VP7 of African horse sickness virus was prepared. An IgM capture enzyme-linked immunosorbent assay for detecting AHS was developed by optimizing the antigen concentration,the second antibody concentration and the serum dilution. Preliminary application of the MAC-ELISA method indicated that the MAC-ELISA with the recombined VP7 protein was successfully developed for detecting antibodies to AHSV with good sensitivity and specifi city.

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