Application Analysis of Microcystis aeruginosa Identification Based on Ribosomal Proteins

JIANG Wen-jing;ZHANG Jun-yi;DU Yang;SUN Li-wei;School of Energy and Environment,Southeast University,Taihu Lake Water Environment Engineering Research Center,Southeast University;Wuxi Environmental Monitoring Centre;

ribosomal protein;;Microcystis aeruginosa;;MALDI-TOF MS;;cyanobacterial bloom

This research is to verify the feasibility of matrix-assisted laser desorption-ionization-time-of-flight mass spectrometry(MALDI-TOF MS)in the identification of mixed and actual cyanobacterial samples by employing ribosomal proteins as biomarkers. Mechanicaldisruption and high speed centrifugation were used as pre-treatments to collect the ribosomal protein fraction of cyanobacterial samples,andthen MALDI-TOF MS was applied to identify Microcystis aeruginosa in samples after proteins in situ crystallization with proteomic analysisbased on 13 ribosomal proteins as biomarkers. The results showed that under the optimal conditions,model strain M. aeruginosa was preciselyidentified in the mixed samples and the detection rates of assigned peaks were all greater than 75% with reasonable errors range except thefourth sample. The lowest biomass detection limit and ratio of M. aeruginosa was 2.88×106 cells and 37%,respectively. Furthermore,M. aeruginosa was identified and detected in the cyanobacterial samples from Taihu Lake and the detection rate of assigned peaks was 76.9%. Inconclusion,MALDI-TOF MS employing ribosomal proteins as biomarkers could be successfully applied to the identification of mixed and actualcyanobacterial samples and will be expected as routine tool in environmental cyanobacteria monitoring.