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Position: Home > Articles > Protective effect of mannitol on Volvariella volvacea mycelia at low temperature Acta Agriculturae Shanghai 2017 (4) 6-9

甘露醇对低温下草菇菌丝的保护效应研究

作  者:
陈明杰;宋晓霞;马丹丹;郭力刚;赵妍;王晨光;余昌霞
单  位:
上海百信生物科技有限公司;上海市农业科学院食用菌研究所上海市农业遗传育种重点开放实验室农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心
关键词:
草菇;恢复生长;基因指纹图谱;低温;甘露醇
摘  要:
以草菇菌丝恢复生长情况和基因指纹图谱方法,研究了采用甘露醇溶液低温保藏草菇菌种的效果。结果表明:不耐受低温的草菇菌种在甘露醇溶液的保护下,能够在4℃保藏后快速恢复生长,且10%甘露醇溶液的低温保藏效果要明显优于20%海藻糖溶液;遗传稳定性试验结果显示,经甘露醇低温保藏的草菇菌种的基因指纹图谱未发生改变。
译  名:
Protective effect of mannitol on Volvariella volvacea mycelia at low temperature
作  者:
CHEN Ming-jie;SONG Xiao-xia;MA Dan-dan;Guo Li-gang;ZHAO Yan;WANG Chen-guang;YU Chang-xia;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Key Laboratory of Southern Edible Fungi Resources and Utilization,Ministry of Agriculture;National Engineering Research Center of Edible Fungi;Shanghai Baixin Biotechnology Company Limited;
关键词:
Volvariella volvacea;;Recovery growth;;Genetic fingerprinting;;Low temperature;;Mannitol
摘  要:
The protective effect of mannitol on Volvariella volvacea strains stored at low temperature was investigated in terms of V. volvacea mycelia recovery growth and genetic fingerprinting. The results showed that under mannitol solution protection the low temperature-intolerant V. volvacea strains could quickly recover and grow after storage at 4 ℃,and the protective effect of 10% mannitol solution was much better than that of 20/%trehalose solution. The test for genetic stability indicated that mannitol didn't change the genetic characteristics of V. volvacea strains.

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