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当前位置: 首页 > 文章 > 槟榔江水牛FSHR基因分离鉴定及其功能初步分析 云南农业大学学报(自然科学) 2018 (6) 1060-1067
Position: Home > Articles > Isolation and Identification of Binglangjiang Buffalo FSHR Gene and Preliminary Analysis on Its Function Journal of Yunnan Agricultural University(Natural Science) 2018 (6) 1060-1067

槟榔江水牛FSHR基因分离鉴定及其功能初步分析

作  者：
周芳廷;杨云;刘丽仙;张永云;苗永旺
单  位：
云南农业大学农科专业基础实验教学示范中心;云南农业大学动物科学技术学院;云南农业职业技术学院畜牧兽医学院
关键词：
槟榔江水牛;FSHR;生物信息学分析;结构与功能
摘  要：
【目的】揭示槟榔江水牛FSHR基因的结构与功能。【方法】采用RT-PCR法克隆了槟榔江水牛FSHR基因的编码区全序列,并利用生物信息学方法对其基因编码产物的理化特性、结构及功能进行了初步分析。【结果】槟榔江水牛FSHR基因编码区全长为2 088 bp,编码695个氨基酸。氨基酸序列比对显示:槟榔江水牛FSHR与其他哺乳动物的同源性在89.4%以上。槟榔江水牛FSHR蛋白N-端含信号肽和7个跨膜结构,属细胞膜疏水蛋白。该蛋白含有7tmA_FSH-R、LRRNT、LRR重复单元和GnHR_trans等4个保守结构域。槟榔江水牛FSHR二级结构主要由α螺旋和无规则卷曲所构成,分别占41.73%和38.71%。FSHR最有可能在细胞的转运和结合过程中发挥功能作用(概率0.827)。【结论】槟榔江水牛FSHR属于G蛋白偶联受体家族,在细胞质中合成后转运到达细胞膜,推测通过与FSH激素结合后激活G蛋白偶联作用,进而促进其精子和卵泡的发育与成熟。
译  名：
Isolation and Identification of Binglangjiang Buffalo FSHR Gene and Preliminary Analysis on Its Function
作  者：
ZHOU Fangting;YANG Yun;LIU Lixian;ZHANG Yongyun;MIAO Yongwang;College of Animal Science and Technology,Yunnan Agricultural University;Faculty of Animal Husbandry and Veterinary Medicine,Yunnan Agricultural College of Vocational Education;Teaching Demonstration Center of the Basic Experiments of Agricultural Majors,Yunnan Agricultural University;
单  位：
ZHOU Fangting%YANG Yun%LIU Lixian%ZHANG Yongyun%MIAO Yongwang%College of Animal Science and Technology,Yunnan Agricultural University%Faculty of Animal Husbandry and Veterinary Medicine,Yunnan Agricultural College of Vocational Education%Teaching Demonstration Center of the Basic Experiments of Agricultural Majors,Yunnan Agricultural University
关键词：
Binglangjiang buffalo;;FSHR;;bioinformatic analysis;;structure and function
摘  要：
[Purposes]In order to reveal the structure and fuction of Binglangjiang buffalo FSHR gene.[Methods]The whole coding sequence(CDS) of the FSHR gene was cloned using RT-PCR method, and the physicochemical properties, structure and fuction of its coding product were analysed preliminarily based on the method of bioinformatics.[ Results] The completed CDS of Binglangjiang buffalo FSHR gene is 2 088 bp in length, encoding a peptide composed of 695 amino acid residues. The amino acid sequence alignments showed that identity is more than 89.4% between Binglangjiang buffalo and other mammals. Binglangjiang buffalo FSHR protein is a hydrophobic protein with one N-terminal signal peptide and seven transmembrane regions, located in cell membrane.This protein contains 7 tmA_FSH-R, LRRNT, LRR and GnHR_trans conservative domains. The secondary structures of buffalo FSHR are mainly consist of alpha helix and random curl, occupied41.73% and 38.71%, respectively. The buffalo FSHR probably function in the process of cell transport and binding(probability 0.827).[Conclusions]Binglangjiang buffalo FSHR belongs to the G protein coupled receptor family, which is synthesized in the cytoplasm and transfered to cell membrane. It is speculated that the buffalo FSHR activates G protein coupling mechanism by binding to FSH hormone, which in turn promotes the development and maturation of buffalo sperm and follicles.

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