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灵发素(LFS)对诱导甘蔗愈伤组织的影响

作  者:
陈念平;蓝桃菊;龚银花;解云英;许鸿章;梁琼月;周凤珏;何冰;许鸿源
单  位:
广西大学农学院;中国医学科学院医药生物技术研究所
关键词:
甘蔗;灵发素;愈伤组织
摘  要:
目的:建立高效率诱导甘蔗优质愈伤组织的技术。方法:以甘蔗幼叶作外植体,MS为基本培养基,分别添加不同浓度的新型诱导剂LFS与传统的诱导剂2,4-D进行对比研究。结果:LFS 0.05~1.00 mg/L均能高效优质地诱导愈伤组织,其适宜浓度为0.20 mg/L,培养20 d,无需更换新鲜培养基,出愈率达98%,褐化、板结轻微,转绿率达16.8%,效果显著优于2,4-D。结论:LFS可以比2,4-D更高效优质地诱导甘蔗愈伤组织,并显著简化生产程序。
译  名:
Influence of LFS on Inducing Sugarcane Callus
作  者:
CHEN Nian-ping;LAN Tao-ju;GONG Yin-hua;XIE Yun-ying;XU Hong-zhang;LIANG Qiong-yue;ZHOU Feng-jue;HE Bing;XU Hong-yuan;Agronomic College of Guangxi University;Guangxi Academy of Agricultural Sciences;Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences;
关键词:
sugarcane;;LFS;;callus
摘  要:
Objective: To establish the technology for inducing efficiently the sugarcane callus with high quality.Methods: Take young leaf cuts of sugarcane as explants,and MS as basic medium added respectively different concentrations of new inductive agent LFS,and with the traditional inductive agent 2,4-D as ck for contrast research. Results: MS + LFS 0. 05- 1. 00 mg / L could all induce callus with high efficient and quality. The suitable concentration of LFS was 0. 20 mg / L,cultivated 20 d,callus induction rate reached 98%,browning and hardening slightly,greenish rate reached 16. 8%. Such callus was very suitable for organ differentiation in further. Conclusion: LFS is better than 2,4-D to induce efficiently sugarcane callus with high quality and simplifing significantly the productive process.

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