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当前位置: 首页 > 文章 > MEAM1和MED两个烟粉虱隐种取食对甘蓝转录组影响的差异 植物保护学报 2021 (6) 1380-1386
Position: Home > Articles > Differences in the transcriptomes of Brassica oleracea leaves infested by MEAM1 and MED cryptic species of tobacco whitefly Bemisia tabaci Journal of Plant Protection 2021 (6) 1380-1386

MEAM1和MED两个烟粉虱隐种取食对甘蓝转录组影响的差异

作  者：
胡杰;卢靖天;付培艺;彭宇;焦晓国;张友军
单  位：
湖北大学生命科学学院生物催化与酶工程国家重点实验室;中国农业科学院蔬菜花卉研究所
关键词：
烟粉虱;转录组分析;基因;苯丙烷合成通路;芥子油苷;实时荧光定量PCR
摘  要：
为绿色防控烟粉虱Bemisia tabaci提供新策略,采用转录组分析MEAM1和MED两个烟粉虱隐种取食后甘蓝差异基因表达情况,并选取差异表达基因最多的甘蓝苯丙烷类通路上PAL2、C4H、4CL1和CHI四个基因进行实时荧光定量PCR测定。结果显示,与对照相比,MEAM1和MED烟粉虱隐种取食后甘蓝差异表达基因数分别为693个和1 030个。GO功能注释和KEGG富集分析显示,差异表达基因主要集中在苯丙烷类生物合成、萜类挥发物合成、芥子油苷合成、类黄酮生物合成和植物激素信号传导途径等与甘蓝抗虫物质合成相关的通路上。虽然MEAM1与MED两个烟粉虱隐种均能显著激活植物激素信号传导途径和苯丙烷类合成通路调控的酚类物质合成途径,但MED烟粉虱隐种与甘蓝互作的差异表达基因数高于MEAM1烟粉虱隐种。MEAM1烟粉虱隐种取食甘蓝12 h后,甘蓝苯丙烷通路上游PAL2、C4H和4CL1三个基因均显著上调,而MED烟粉虱隐种取食后,甘蓝苯丙烷通路上PAL2、C4H、4CL1和CHI四个基因均变化不显著,这4个差异基因的表达模式与测序结果一致,表明测序结果的可信度高。
译  名：
Differences in the transcriptomes of Brassica oleracea leaves infested by MEAM1 and MED cryptic species of tobacco whitefly Bemisia tabaci
作  者：
Hu Jie;Lu Jingtian;Fu Peiyi;Peng Yu;Jiao Xiaoguo;Zhang Youjun;State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences;
关键词：
Bemisia tabaci;;transcriptome analysis;;gene;;phenylpropanoid biosynthesis pathway;;glucosinolates;;quantitative real-time PCR
摘  要：
In order to provide a novel strategy for green control of tobacco whitefly Bemisia tabaci,transcriptome analysis was used to analyze the differentially expressed genes(DEGs) of cabbage after being infested by MEAM1 and MED cryptic species of B. tabaci. Four genes PAL2, C4H, 4 CL1 and CHI involved in the phenylpropanoid biosynthesis pathway with the most DEGs were selected for quantitative real-time PCR(qRT-PCR) to verify the reliability of cabbage transcriptome data. The results showed that, compared with the control, the numbers of DEGs in cabbages after being infested by MEAM1 and MED were 693 and 1 030, respectively. GO functional annotation and KEGG enrichment analysis showed that the DEGs were mainly concentrated in the pathways related to the synthesis of insect resistant substances in cabbage, such as phenylpropanoid biosynthesis, terpenoid biosynthesis,glucosinolate biosynthesis, flavonoid biosynthesis and plant hormone signal transduction pathway. Although the plant hormone signal transduction pathway and phenylpropanoid biosynthesis pathway were significantly activated in both MEAM1 and MED, the number of DEGs in cabbage infested by MED was higher than that by MEAM1. After cabbage being infested by MEAM1 for 12 h, the three genes PAL2, C4H and 4 CL1 upstream of the phenylpropanoid biosynthesis pathway in cabbage were significantly up-regulated, while PAL2, C4H, 4 CL1 and CHI in cabbage infested by MED did not change significantly. The expression patterns of these four DEGs analyzed by qRT-PCR were consistent with those by transcriptome sequencing, indicating that the transcriptome data were highly reliable.

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