Rapid Detection of Drug Resistance of Escherichia coli and Salmonella Isolates from Animals

Zhu Xiao-ling,Du Jia-fa,Cai Ya-na,Hu Ming,Bai hua,Qi Jing,Liu Yu-qing(1.Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences,Jinan 250100,China;2.Veterinary Faculty Hunan Agricultural University,Changsha 410128,China;3.Veterinary Faculty,Qingdao Agricultural University,Qingdao 266109,China)

96 dot-matrix antibiotic susceptibility testing kit;Escherichia coli(E.coli)i;Salmonella;Quinolones(QNS);Multi-drug resistance

The drug susceptibility of Escherichia coli and Salmonella from poultry and swine in Shandong was detected by new type 96 dot-matrix antibiotic susceptibility testing kit.Experimental results showed that the resistance rates of Escherichia coli to AMP and QNS reached over 98%,the resistance rate of Escherichia coli from poultry was higher than from swine and the resistance spectrum of Escherichia coli from swine was wider than from poultry.The resistance rate of Salmonella was lower than Escherichia coli,but presented multiple resistanta-nce.The 96 dot-matrix antibiotic susceptibility testing kit was simple,exact,fast,large-flux and frugal and was very suitable to large-scale drug resistance monitoring in the laboratory.The new tool provided technical support for antibiotics application and the treatment of bacterial disease,thus avoiding blind use of antibiotics and reducing the drug resistancerate.