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当前位置: 首页 > 文章 > 微卫星DNA鉴别克隆香鱼 水产学报 2003,27 (4) 295-299
Position: Home > Articles > Identification of clonal Plecoglossus altivelis using microsatellite DNA marker Journal of Fisheries of China 2003,27 (4) 295-299

微卫星DNA鉴别克隆香鱼

作  者：
董仕;谷口顺彦
单  位：
东北大学大学院农学研究科;天津师范大学化学与生命科学学院
关键词：
香鱼;克隆;微卫星DNA
摘  要：
实验应用微卫星DNA检测技术,分析了Ⅰ至Ⅶ组共42尾香鱼的Pal-1 和Pal-2 两个基因座位的基因型。结果显示Ⅱ、Ⅲ、Ⅳ和Ⅶ组共22尾在两个基因座位上均为纯合,是基因型完全相同的克隆鱼,并且Ⅶ组10尾为人工诱导成功的雌核发育二倍体,而Ⅰ为混入本克隆的其它类型的香鱼;Ⅴ组9尾为Ⅰ与海产香鱼的子代;Ⅵ组10尾为Ⅱ或Ⅲ与海产香鱼的子代。
译  名：
Identification of clonal Plecoglossus altivelis using microsatellite DNA marker
作  者：
DONG Shi~1, TANIGUCHI Nobuhiko~2(1.Department of Biology, Tianjin Normal University, Tianjin300074, China; 2.Graduate School of Agricultural Science, Tohoku University, Miyagi 981-8555, Japan)
关键词：
Plecoglossus altivelis; clone; microsatellite DNA
摘  要：
The specimen used in this study was 42 fish of seven groups of ayu,Plecoglossus altivelis, and they should be one clone induced artificially or samples obtained from the eggs of amphidromous form inseminated by the sperm of the individuals of the same clone. The genomic DNA were extracted from caudal fin of adult or all fish of larva and microsatellite DNA were amplified by PCR. The genotypes of individuals were identified according to electrophoretic patterns of the products of PCR. The results suggest 22 fish of Ⅱ, Ⅲ, Ⅳ and Ⅶ groups be one clone,because they have the same genotypes at two loci of Pal-1~*and Pal-2~* respectively. 10 fish of Ⅶ group were gynogenetic diploids induced successfully and one clone. 1 fish of Ⅰ is other type fish. 19 fish of Ⅴ and Ⅵ groups were offspring of Ⅰ, Ⅱ or Ⅲ and amphidromous form.

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