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Position: Home > Articles > Isolation,identification and drug sensitivity of Pseudomonas aeruginosa in foxes Animal Husbandry & Veterinary Medicine 2018 (1) 99-102

狐源绿脓杆菌的分离鉴定及药敏试验

作  者:
李巧玲;庞洪泽;张文举;张召兴;吉志新;张志强;吴同垒;李蕴玉;史秋梅;贾青辉;李佩国
单  位:
河北科技师范学院/河北省预防兽医重点实验室
关键词:
狐狸;绿脓杆菌;致病性;16S rRNA;药敏试验
摘  要:
为了鉴定引起狐狸肺炎的病原菌,采用常规鉴定法和16S rRNA PCR方法从肺炎的狐狸肺脏病料组织中进行分离培养与鉴定,确定该菌为绿脓杆菌(Pseudomonas aeruginosa)。该菌的16S rRNA基因的系统发育树分析表明,分离菌与绿脓杆菌同源性达100%。动物致病性试验结果表明该分离菌株有致病性。药敏试验显示分离菌株对头孢曲松、阿米卡星、左氧氟沙星、阿奇霉素等药物敏感。
译  名:
Isolation,identification and drug sensitivity of Pseudomonas aeruginosa in foxes
作  者:
LI Qiaoling#;PANG Hongze#;ZHANG Wenju;ZHANG Zhaoxing;JI Zhixin;ZHANG Zhiqiang;WU Tonglei;LI Yunyu;SHI Qiumei;JIA Qinghui;LI Peiguo;Key Laboratory of Preventive Veterinary Medicine in Heibei Province,Hebei Normal University of Science and Technology;
关键词:
fox;;Pseudomonas aeruginosa;;pathogenicity;;16S rRNA;;drug sensitivity
摘  要:
To identify the pathogen causing serious pneumonia in foxes,one bacterial strain was isolated from lung tissue samples and identified by bacteria characteristics and 16 S rRNA gene amplification.The result showed that the isolated strain was Pseudomonas aeruginosa.The 16 S rRNA gene phylogenetic tree showed that the sequence of the isolate strain had 100% homology with the P.aeruginosa reference strains.Animal experiment showed that the isolated strain was pathogenic.Drug sensitivity showed that the isolate strain was highly sensitive to ceftriaxone,amikacin,levofloxacin and azithromycin.

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