作 者：
赵世文;杨金龙;达剑森;袁燕;邹辉;顾建红;刘学忠;卞建春;刘宗平
单 位：
江苏省动物重要疫病与人兽共患病防控协同创新中心;扬州市邗江区畜牧兽医站;扬州大学兽医学院
关键词：
亚慢性;铅;镉;α-硫辛酸;SD大鼠;肝功能
摘 要：
为了研究铅镉联合暴露对大鼠肝功能的影响以及α-硫辛酸的保护作用,试验以300 mg/L铅(Pb)、50 mg/L镉(Cd)单独或联合(Pb+Cd)采用自由饮水方式染毒SD大鼠,α-硫辛酸保护组在Pb、Cd自由饮水的同时按体重以50 mg/kgα-硫辛酸灌胃,12周后测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、血清碱性磷酸酶(ALP)活性及总蛋白(TP)、白蛋白(ALB)的含量。结果表明:与对照组相比,铅组、镉组ALT活性极显著升高(P<0.01),AST和ALP活性极显著下降(P<0.01),镉组TP和ALB含量显著或极显著下降(P<0.05或P<0.01),铅组TP和ALB含量无显著性差异,铅镉联合组ALT和AST活性极显著升高(P<0.01),ALP活性、TP和ALB含量无显著性差异;与铅、镉单独染毒组相比,铅镉联合组ALT、AST活性极显著升高(P<0.01),ALP活性、TP和ALB含量无显著性差异;与相应染毒组相比,α-硫辛酸保护组大鼠肝功能各项指标均有所恢复,部分具有统计学意义(P<0.05)。说明铅、镉单独或联合暴露对大鼠肝功能有损伤作用,α-硫辛酸可在一定程度上降低铅、镉单独或联合引起的肝功能损伤。
译 名：
The effect of the combined exposure of sub-chronic lead and cadmium on liver function of rats and the protection effect of α-lipoic acid
作 者：
ZHAO Shiwen;YANG Jinlong;DA Jiansen;YUAN Yan;ZOU Hui;GU Jianhong;LIU Xuezhong;BIAN Jianchun;LIU Zongping;College of Veterinary Medicine,Yangzhou University;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses;Hanjiang Station of Animal Husbandry and Veterinary Medicine of Yangzhou;
关键词：
sub-chronic;;lead;;cadmium;;α-lipoic acid;;SD rat;;liver function
摘 要：
To study the effect of the combined exposure of sub- chronic lead and cadmium on liver function of rats and the protection effect of α- lipoid- acid,SD rats were exposed to 300 mg/L of lead( Pb) and 50 mg/L of cadmium( Cd) alone or in combination by free drinking water. Meanwhile the rats in the α- lipoic acid- protected group were fed 50 mg/Kg·Bw of α- lipoid- acid by intragastric administration,and then the activities of serum alanine aminotransferase( ALT),aspartate aminotransferase( AST) and the contents of serum alkaline phosphatase( ALP),total protein( TP) and albumin( ALB) were determined after 12 weeks. The results showed that the activities of ALT were significantly increased( P < 0. 01) in the Pb- or Cd- treated groups compared with the control group,and the activities of AST and ALP were significantly decreased( P < 0. 01) in the Pb- or Cd- treated groups; the contents of TP and ALB were significantly decreased( P < 0. 05) or highly significantly decreased( P < 0. 01) in the Cd- treated group,but the contents of TP and ALB had no significance( P > 0. 05) in the Pb- treated group; the activities of ALT and AST were highly significantly increased significantly( P < 0. 01) in the Pb and Cd co- treated group,but the ALP activity and the contents of TP and ALB had no significances( P > 0. 05) in the Pb and Cd co- treated group. The activities of AST and ALP increased significantly( P < 0. 01) in the Pb and Cd co- treated group compared with the Pb or Cd- treated groups,but the ALP activity and the contents of TP and ALB had no significances( P > 0. 05) in the Pb and Cd co- treated group; The various indexes of liver function in the α- lipoic acid- protected group were recovered and some of them were statistically significant( P < 0. 05) compared with the corresponding exposure group. The results indicate that sub- chronic Pb and Cd alone or combined exposure has damage effect on the liver function of rats,while and α- lipoic acid can reduce the damage caused by Pb and Cd alone or combined exposure to some degree.
