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Position: Home > Articles > Study on the activities of digestive enzyme and anti-oxidative enzymes in juvenile sea urchin(Strongylocentrotus intermedius) Feed Industry 2016,37 (22) 26-29

中间球海胆消化酶和抗氧化酶活力的研究

作  者:
左然涛;侯受权;李广;常亚青
单  位:
大连海洋大学农业部北方海水增养殖重点实验室
关键词:
中间球海胆;胃蛋白酶;淀粉酶;脂肪酶;超氧化物歧化酶;总抗氧化力;过氧化氢酶
摘  要:
为了分析中间球海胆的食性及基本生理特性,研究以中间球海胆幼胆为研究对象,测定了养殖条件下摄食海带海胆消化道中消化酶种类、活力及体腔液中抗氧化酶的活力。结果显示:1中间球海胆消化道中具有胃蛋白酶、脂肪酶和淀粉酶,其活力分别为(7.72±0.92)、(17.71±1.37)U/mg pro和(1.98±0.31)U/mg pro。2体腔液中超氧化物歧化酶(SOD)、总抗氧化力(AOC)和过氧化氢酶(CAT)活力分别为(20.36±0.62)、(2.29±0.44)、(8.08±2.02)U/ml。结果表明:中间球海胆对蛋白质、淀粉和脂肪具有较强消化能力且具有较强抗氧化能力,这为中间球海胆专用配合饲料的开发提供了理论依据,同时有助于通过营养手段调控其抗应激能力。
译  名:
Study on the activities of digestive enzyme and anti-oxidative enzymes in juvenile sea urchin(Strongylocentrotus intermedius)
作  者:
Zuo Rantao;Hou Shouquan;Li Guang;Chang Yaqing;
关键词:
Strongylocentrotus intermedius;;pepsin;;amylase;;lipase;;superoxide dismutase;;T-AOC;;catalase
摘  要:
This study was conducted to investigate the categories and activity of digestive enzymes in digestive tracts, as well as activity of anti-oxidative enzymes in coelomic fluid of juvenile sea urchin(Strongylocentrotus intermedius). Results showed that: 1 Pepsin, lipase and amylase exist in the digestive tract of sea urchin. Among them, activity of lipase was highest(17.71±1.37) U/mg pro, followed bypepsin(7.72±0.92) U/mg pro and amylase(1.98±0.31) U/mg pro. 2 Superoxide dismutase, anti-oxidative capacity and catalase in coelomic fluid of juvenile sea urchin were(20.36±0.62),(2.29±0.44),(8.08±2.02) U/ml, respectively. These results showed that juvenile sea urchin had digestive enzyme activity and anti-oxidative capacity. This study will contribute to developing formulated feeds and regulating anti-oxidative capacity of sea urchin by altering feed formulations.

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