Position: Home > Articles > Molecular cloning and polymorphism of the pig Myl1 promoter
Guangdong Agricultural Sciences
2009
(6)
127-130
猪Myl1基因启动子克隆及多态性研究
作 者：
凌飞;李加琪;钟万福;杜红丽;陈瑶生
单 位：
华南理工大学生物与工程学院;华南农业大学动物科学学院;中山大学生命科学学院
关键词：
肌球蛋白轻链1基因;猪BAC文库;启动子;单核苷酸多态性
摘 要：
肌球蛋白轻链1基因(Myl1)编码的不同剪切体(主要是MLC1f和MLC3f)与哺乳动物骨骼肌肌纤维类型相关。通过猪BAC文库筛选及引物步行法测序获得Myl1基因的启动子序列,采用PCR-RFLP技术分析蓝塘猪、大花白猪、大白猪、长白猪和杜洛克猪5个品种Myl1基因启动子区的多态性。结果表明,Myl1基因-678C/T位点在蓝塘和大花白等本地猪种中多态性丰富,而在杜洛克、长白和大白等外来猪种中几乎只有CC基因型。
译 名：
Molecular cloning and polymorphism of the pig Myl1 promoter
作 者：
LING Fei1,2,LI Jia-qi2,ZHONG Wan-fu2,DU Hong-li1,CHEN Yao-sheng3(1.School of Biological Science and Engineering,South China University of Technology,Guangzhou 510006,China;2.College of Animal Science,South China Agricultural University,Guangzhou 510642,China;3.School of Life Science,Sun Yat-Sen University,Guangzhou 510006,China)
关键词：
Myl1 gene;pig BAC library;promoter;SNP
摘 要：
Distinctive alternative variants of Myl1(MLC1f and MLC3f) were found to be associated with the skeletal muscle fiber type of mammalian.The 5'-flanking region of the Myl1 gene was isolated from a pig BAC library and was sequenced by primer-walking method,which was used to analyse polymorphism of breeds(40 LanTang pigs,40 DaHuaBai pigs,80 landrace pigs,80 large white pigs and 50 duroc pigs) with PCR-RFLP.The results showed that the SNP of-678C/T was identified in the Myl1 promoter.Distribution of genotype in the Chinese indigenous pig was higher than the exotic pig,and TT genotype was not detected in exotic breeds.
导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
