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当前位置: 首页 > 文章 > 3种楠木DNA提取及其ISSR标记鉴别 四川农业大学学报 2016,34 (4) 450-455+477
Position: Home > Articles > DNA Extraction and Wood ISSR Identification from Three Phoebe Species Journal of Sichuan Agricultural University 2016,34 (4) 450-455+477

3种楠木DNA提取及其ISSR标记鉴别

作  者：
王英;骆嘉言;钟文翰;石江涛;樊帅
单  位：
南京林业大学材料科学与工程学院;浙江省家具检测技术研究重点实验室
关键词：
紫楠;浙江楠;桢楠;ISSR-PCR;分子鉴定
摘  要：
【目的】采用ISSR分子标记鉴别楠属3个树种。【方法】以紫楠、浙江楠和桢楠木材为对象,比对改良CTABSDS法、CTAB法和试剂盒法3种方法提取的木材基因组DNA质量,采用ISSR-PCR技术对3个树种24个样本基因组DNA进行扩增。【结果】改良CTAB-SDS法和CTAB法较适合这3种木材的DNA提取。序列扩增筛选出7条稳定、条带清晰且多态性好的引物,确定其最佳退火温度。7条引物共扩增出67条大小为100~2 000 bp的条带,其中多态性条带43条,多态率64.2%。【结论】这7条引物扩增的多态性条带都可用于鉴别与区分这3种楠木。
译  名：
DNA Extraction and Wood ISSR Identification from Three Phoebe Species
作  者：
WANG Ying;LUO Jia-yan;ZHONG Wen-han;SHI Jiang-tao;FAN Shuai;College of Materials Science and Engineering,Nanjing Forestry University;Key Laboratory of Furniture Inspection Technology of Zhejiang Province;
关键词：
P.sheareri;;P.chekiangensis;;P.zhennan;;ISSR-PCR;;molecular identification
摘  要：
【Objective】The aim of the study was to identifying 3 phoebe species by inter simple sequence repeat(ISSR)technique.【Method】The optimized CTAB-SDS,CTAB and kit for DNA extraction methods were used to extract wood DNA from P. sheareri,P. chekiangensis and P. zhennan. Three species were amplified with ISSR-PCR technique.【Results】 The optimized CTAB-SDS and CTAB methods were better for DNA extraction of 3 woods. 7 ISSR primers were screened and their amplification were stable,clear and high polymorphic. A total of 67 bands were obtained through 7 primers,43,64.2% of which were polymorphic. DNA fragment size were ranged from 100 bp to 2 000 bp.【Conclusion】These polymorphism bands amplified by the 7 primers can distinguish the woods of 3species.

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