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当前位置: 首页 > 文章 > 四川鸽毛滴虫病调查和鸽毛滴虫体外保存方法 西南农业学报 2009,22 (1) 192-196
Position: Home > Articles > Investigation of Trichomonas columbae Disease for pigeon in Sichuan Province and Research on in Vitro Preservative Method of T. columbae Body Southwest China Journal of Agricultural Sciences 2009,22 (1) 192-196

四川鸽毛滴虫病调查和鸽毛滴虫体外保存方法

作  者：
黄名英;廖党金;傅安静
单  位：
成都农业科技职业学院;西南民族大学动物科技学院;四川省畜牧科学研究院
关键词：
鸽;鸽毛滴虫;保存方法
摘  要：
采用随机抽样和镜检虫体法,调查了3个养鸽场和2个市场上的鸽毛滴虫病,在17000只肉鸽中,随机抽查1980只,鸽毛滴虫病感染率为75.12%,感染率的范围为34.44%~94.00%;信鸽50只,毛滴虫病的感染率为0%。为探索鸽毛滴虫的保存方法,就保存温度、保存液种类、保存液的pH值、保存液中的鸽毛滴虫密度、冷冻保护剂等对鸽毛滴虫的影响进行了测定,结果表明,保存溶液为生理盐水或pH7.2的PBS液、温度为28℃左右、溶液pH为7.2、保存液中的鸽毛滴虫密度为显微镜以10×40倍下每个视野50个~200个虫体为佳。
译  名：
Investigation of Trichomonas columbae Disease for pigeon in Sichuan Province and Research on in Vitro Preservative Method of T. columbae Body
作  者：
HUANG Ming-ying1,3,LIAO Dang-jin2,FU An-jing3(1.Southwestern University for Nationalites,Sichuan Chengdu 610066,China;2.Sichuan Academy of Animal Sciences,Sichuan Chengdu 610066,China;3.Chengdu Vocational Academy of Agricultural Science & Technology,Sichuan Chengdu 611130,China)
关键词：
Pigeon;Trichomonas columbae;in Vitro preservative method
摘  要：
The epidemic situations of Trichomonas columbae disease at 3 pigeon farms were investigated using the method of examining worm under microscope.A total number of 2030 pigeons randomLy selected from 17 000 were examined.The results showed the infective rate was 75.12 % and the range of infective rate were 34.44 %-94.00 % at the 3 pigeon farms in Sichuan province.To obtain a in vitro preservative method,temperature,kind and pH of preservative liquid,density of T.columbae body in liquid and coolant affecting the parasite were measured.The results were that the in vitro preservative condition of T.columbae was the solution of 0.9 % sodium chloride or pH7.2 PBS,the temperature of about 28℃,the pH of 7.2 and the density of T.columbae body in liquid of 50-200 bodies per field of microscope with 10×40 times.

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