Position: Home > Articles > UV-Vis Spectrophotometric Determination of Total Isoflavones in Polyalthia plagioneura Diels
Hubei Agricultural Sciences
2012,51
(22)
223-225
紫外-可见分光光度法测定斜脉暗罗总异黄酮含量
作 者：
刘冰晶;曾靖;江智伟;李超;宋鑫明
单 位：
赣南医学院药学院/江西省脑血管药理重点实验室;海南师范大学化学与化工学院/“省部共建”热带药用植物教育部重点实验室
关键词：
斜脉暗罗(Polyalthia plagioneura Diels);总异黄酮;紫外-可见分光光度法
摘 要：
以染料木素为标准品,采用紫外-可见分光光度法在266 nm处测定斜脉暗罗(Polyalthia plagioneu-ra Diels)茎、叶中总异黄酮的含量。结果表明染料木素标准品在0.20～1.60μg/mL范围内呈良好线性关系,回归方程为C=0.003 9A,R=0.999 9。斜脉暗罗茎和叶中的总异黄酮含量分别为0.055 4%和0.065 3%。此法简便、准确,重复性好,可用作斜脉暗罗中总异黄酮的含量测定。
译 名：
UV-Vis Spectrophotometric Determination of Total Isoflavones in Polyalthia plagioneura Diels
作 者：
LIU Bing-jing1,ZENG Jing1,JIANG Zhi-wei1,LI Chao1,SONG Xin-ming2(1.Jiangxi Provincical Key Lab of Cerebrovascular pharmacology/College of Pharmacy,Gannan Medical University,Ganzhou 341000,Jiangxi,China;2.Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education/College of Chemistry and Chemical Engineering,Hainan Normal University,Haikou 571158,China)
关键词：
Polyalthia plagioneura Diels;total isoflavones;UV-Vis spectrophotometric method
摘 要：
The content of isoflavones in the stem and leaf of Polyalthia plagioneura Diels was determined by UV-Vis spectrophotometric method with genistein as the standard.The results showed that the calibration curve was linear(R=0.999 9) in the range of 0.000 5～0.004 5 μg/mL,and the regression equation was C=0.003 9A.The content of total isoflavones in stem and leaf was 0.055 4% and 0.065 3% respectively.This method was simple,accurate,reproducible and suitable for the determination of total isoflavones in the stem and leaf of P.plagioneura Diels.
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