Position: Home > Articles > Cloning of Biosynthetic Gene Cluster of Ectoine from Streptomyces mutabilis TRM 45540
Acta Agriculturae Boreali-occidentalis Sinica
2017,26
(3)
471-476
易变链霉菌TRM 45540四氢嘧啶合成相关基因的克隆
作 者：
张慧艳;刘芝瑾;徐同;马金福;梁凯;罗晓霞
单 位：
新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室/塔里木大学生命科学学院
关键词：
易变链霉菌;四氢嘧啶;基因克隆
摘 要：
为了探索嗜盐放线菌对高盐环境的适应机理,以分离于罗布泊易变链霉菌TRM 45540为材料,利用分子克隆技术,构建四氢嘧啶异源生物合成工程菌(以E.coli为宿主),对ectA、ectB、ectC与ectABC基因进行异源表达,分析异源宿主菌的耐盐能力。结果表明:TRM 45540四氢嘧啶合成相关基因ectA为474bp,预测编码蛋白为19ku;ectB为1 272bp,预测编码蛋白为44.6ku;ectC为399bp,预测编码蛋白为19ku;全长基因ectABC为2 403bp,并且携带有ectABC基因工程菌的耐盐能力显著提高。
译 名：
Cloning of Biosynthetic Gene Cluster of Ectoine from Streptomyces mutabilis TRM 45540
作 者：
ZHANG Huiyan;LIU Zhijin;XU Tong;MA Jinfu;LIANG Kai;LUO Xiaoxia;Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production&Construction Corps/College of Life Science,Tarim University;
关键词：
Streptomyces mutabilis;;Ectoine;;Gene clone
摘 要：
The aim of this study is to conduct research into adaptive mechanism of halophile actinomycetes in hypersaline environments.The wide type strain Streptomyces mutabilis TRM 45540 was used as material,we inserted the ectA,ectB,ectCand ectABCinto the expression vector pET28 ato generate recombinant plasmids ectABC+pET-28a/BL21(DE3),then analyzed the salt tolerance of recombinants.The results showed that ectABCgene was 2 403 bp,including three subunits such as ectA,ectB and ectC.EctAgene was 474 bp,the encoding protein 19 ku,ectBgene 1 272 bp,the encoding protein44.6ku;ectCgene was 399 bp,the encoding protein was 19 ku.Moreover,the recombinant showed higher salt tolerance.
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